SPHK1 Antibody

• Catalog number
  F50216-0.08ML
• Price
  Please ask
• Size
  0.08 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  SPHK1
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, ELISA
• Recommended dilutions
  Western blot: 1:500
• Notes
  Titration of the SPHK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This SPHK1 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q9NYA1
• Purity
  Purified
• Description
  Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.
• Immunogen
  A portion of amino acids 1-30 from the human protein was used as the immunogen for this SPHK1 antibody.
• Storage
  Aliquot the SPHK1 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  SPHK1  
• Gene symbol
  SPHK1
• Short name
  Anti-SPHK1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to SPHK1
• Alternative technique
  antibodies
• Alternative to gene target
  sphin this GO sine kinase 1, SPHK, SPHK1 and IDBG-69650 and ENSG00000176170 and 8877, protein phosphatase 2A binding, nuclei, Sphk1 and IDBG-214470 and ENSMUSG00000061878 and 20698, SPHK1 and IDBG-643068 and ENSBTAG00000008507 and 618605

Gene info

• Identity
  HGNC:11240
• Gene
  SPHK1
• Long gene name
  sphingosine kinase 1
• Synonyms
  • SPHK
• GenBank acession
  • BC030553
• Locus
  17q25.1
• Discovery year
  1999-02-01
• Entrez gene record
  8877
• Pubmed identfication
  • 9726979
• RefSeq identity
  • NM_182965
  • NM_021972
• VEGA ID
  OTTHUMG00000180177

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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