SPHK1 Antibody
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Catalog numberF40149-0.08ML
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PricePlease ask
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Size0.08 ml
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CategoryAntibody
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ConcentrationIn 1X PBS pH 7.4 with 0.09% sodium azide
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FormPurified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenSPHK1
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB, FACS, IHC, ELISA
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Recommended dilutionsWestern blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
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NotesTitration of the SPHK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis SPHK1 antibodyis to be used only for research purposes and not for diagnostics..
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UniprotQ9NYA1
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PurityPurified
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DescriptionSPHK1 is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.
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ImmunogenA portion of amino acids 313-343 from the human protein was used as the immunogen for this SPHK1 antibody.
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StorageAliquot the SPHK1 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolSPHK1
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Short nameAnti-SPHK1
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit Ig
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Alternative nameAntibodies to SPHK1
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Alternative techniqueantibodies
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Alternative to gene targetsphin this GO sine kinase 1, SPHK, SPHK1 and IDBG-69650 and ENSG00000176170 and 8877, protein phosphatase 2A binding, nuclei, Sphk1 and IDBG-214470 and ENSMUSG00000061878 and 20698, SPHK1 and IDBG-643068 and ENSBTAG00000008507 and 618605
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Gene info
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Identity
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Gene
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Long gene namesphingosine kinase 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-02-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data