PF4 Antibody / Platelet factor 4
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Catalog number
R32448
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Price
Please ask
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Size
0.1mg
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Category
Antibody
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Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
PF4 / Platelet factor 4
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB, ELISA (protein)
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Recommended dilutions
Western blot: 0.5-1ug/ml,ELISA: 0.1-0.5ug/ml (human protein tested); request BSA-free format for coating
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Added buffer
Lyophilized from 1X Phosphate Buffered Saline (PBS) with 2.5% BSA and 0.025% sodium azide
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Intented use
This Platelet factor 4 antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
P02776
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Purity
Antigen affinity
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Description
Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). By in situ hybridization, the CXCL4 gene is mapped to chromosome 4q12-q21. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.
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Immunogen
Amino acids E32-S101 were used as the immunogen for the Platelet factor 4 antibody.
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Storage
Prior to reconstitution, store at 4oC. After reconstitution, the Platelet factor 4 antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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Localization
Cytoplasmic, membranous
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Additional description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. Platelets, also called thrombocytes or cloth cells in blood and are needed to stop bleeding by clumping and clotting the blood the vessels when the an injury occurs. Teh bone marrow will produce the platelets that have no nucleus. Platelates are unique to mammals, the are curved shaped 1900nm to 3100 nm large nucleus free clothing structures.
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French translation
anticorps
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Gene target
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Gene symbol
PF4, PPBP
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Short name
Anti- PF4 / Platelet factor 4
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit IgG
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Alternative name
Antibodies to PF4 / Platelet factor 4
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Alternative technique
antibodies
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Alternative to gene target
platelet factor 4, CXCL4 and PF-4 and SCYB4, PF4 and IDBG-24097 and ENSG00000163737 and 5196, CXCR3 chemokine receptor binding, Extracellular
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Gene info
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Identity
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Gene
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Long gene name
platelet factor 4
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2001-06-22
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Entrez gene record
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Pubmed identfication
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VEGA ID
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Locus Specific Databases
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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