PF4 Antibody / Platelet factor 4

• Catalog number
  R32449
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  PF4 / Platelet factor 4
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P, ELISA (protein)
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (FFPE): 1-2ug/ml,ELISA: 0.1-0.5ug/ml (mouse protein tested); request BSA-free format for coating
• Added buffer
  Lyophilized from 1X Phosphate Buffered Saline (PBS) with 2.5% BSA and 0.025% sodium azide
• Intented use
  This PF4 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q9Z126
• Purity
  Antigen affinity
• Description
  Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). By in situ hybridization, the CXCL4 gene is mapped to chromosome 4q12-q21. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.
• Immunogen
  Amino acids V30-S105 were used as the immunogen for the PF4 antibody.
• Storage
  Prior to reconstitution, store at 4oC. After reconstitution, the PF4 antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Localization
  Cytoplasmic, membranous
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Additional description
  Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. Platelets, also called thrombocytes or cloth cells in blood and are needed to stop bleeding by clumping and clotting the blood the vessels when the an injury occurs. Teh bone marrow will produce the platelets that have no nucleus. Platelates are unique to mammals, the are curved shaped 1900nm to 3100 nm large nucleus free clothing structures.
• French translation
  anticorps

• Gene target
  PF4   Platelet   factor  
• Gene symbol
  PF4, PPBP
• Short name
  Anti- PF4 / Platelet factor 4
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to PF4 / Platelet factor 4
• Alternative technique
  antibodies
• Alternative to gene target
  platelet factor 4, CXCL4 and PF-4 and SCYB4, PF4 and IDBG-24097 and ENSG00000163737 and 5196, CXCR3 chemokine receptor binding, Extracellular

Gene info

• Identity
  HGNC:8861
• Gene
  PF4
• Long gene name
  platelet factor 4
• Synonyms
  • SCYB4
  • CXCL4
• Synonyms name
  • chemokine (C-X-C motif) ligand 4
• GenBank acession
  • M25897
• Locus
  4q13.3
• Discovery year
  2001-06-22
• Entrez gene record
  5196
• Pubmed identfication
  • 3622011
• VEGA ID
  OTTHUMG00000130009
• Locus Specific Databases
  • LRG_636

Gene info

• Identity
  HGNC:9240
• Gene
  PPBP
• Long gene name
  pro-platelet basic protein
• Synonyms gene
  • THBGB1
• Synonyms
  • SCYB7
  • TGB
  • NAP-2-L1
  • LA-PF4
  • MDGF
  • LDGF
  • Beta-TG
  • CTAP3
  • CXCL7
  • PBP
  • b-TG1
  • TGB1
  • CTAPIII
  • NAP-2
• Synonyms name
  • platelet basic protein
  • beta-thromboglobulin
  • connective tissue-activating peptide III
  • neutrophil-activating peptide-2
  • chemokine (C-X-C motif) ligand 7
• GenBank acession
  • M54995
• Locus
  4q13.3
• Discovery year
  1991-05-21
• Entrez gene record
  5473
• Pubmed identfication
  • 1826003
• RefSeq identity
  • NM_002704
• Classification
  • Receptor ligands
• VEGA ID
  OTTHUMG00000130008

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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