Peroxiredoxin 4 Antibody (PRDX4)
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Catalog number
R30935
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Price
Please ask
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Size
0.1mg
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Category
Antibody
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Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
Peroxiredoxin 4 (PRDX4)
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB
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Recommended dilutions
Western blot: 0.5-1ug/ml
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Notes
The stated application concentrations are suggested starting amounts. Titration of the Peroxiredoxin 4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This Peroxiredoxin 4 antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
Q13162
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Purity
Antigen affinity
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Description
Peroxiredoxin 4 is an antioxidant enzyme and belongs to the peroxiredoxin family. Functional analysis showed that PRDX4 protects glutamine synthetase from inactivation. Yeast 2-hybrid, immunoprecipitation, and immunoblot analyses indicated that PRDX4 and PRDX1 are capable of homodimerization and heterodimerization with each other but not with the mitochondrial PRDX3. Gel mobility shift and immunoblot analysis found that PRDX4 depletes NFKB binding activity together with a reduction in the amounts of p50, p65, and phosphorylated IKBA, as well as a reduction in the expression of HIV-1 viral proteins. Expression of PRDX4, alone or with PRDX1, increased the resistance of yeast cells to oxidant-induced toxicity. Jin et al.(1997) suggested Peroxiredoxin 4 modulates IKBA phosphorylation in the cytoplasm and thus affects a peroxiredoxin-dependent redox step.
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Immunogen
An amino acid sequence from the C-terminus of human PRDX4 (SETIIPDPAGKLKYFDKLN) was used as the immunogen for this Peroxiredoxin 4 antibody (100% homologous in human, mouse and rat).
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Storage
After reconstitution, the Peroxiredoxin 4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
PRDX4
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Short name
Anti-Peroxiredoxin 4 (PRDX4)
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit IgG
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Alternative name
Antibodies to Peroxiredoxin 4 (PRDX4)
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Alternative technique
antibodies
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Gene info
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Identity
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Gene
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Long gene name
peroxiredoxin 4
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Synonyms
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GenBank acession
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Locus
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Discovery year
2001-11-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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