p63 Antibody / Tumor protein 63
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Catalog number
V3367-20UG
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Price
Please ask
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Size
20 ug
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Category
Antibody
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Concentration
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
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Form
Purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
p63 / Tumor protein 63
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens). Others not known. ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
FACS, IF, IHC-P
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Recommended dilutions
Flow Cytometry: 0.5-1ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
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Notes
The optimal dilution of the p63 antibody for each application should be determined by the researcher.
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Intented use
This p63 antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
Q9H3D4
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Purity
Purified by protein A Affinity Chromatography
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Description
p63 is a homolog of the tumor suppressor p53. It is identified in basal cells in the epithelial layers of a variety of tissues, including epidermis, cervix, urothelium, breast and prostate. p63 was detected in nuclei of the basal epithelium in normal prostate glands; however, it was not expressed in malignant tumors of the prostate. As a result, p63 has been reported as a useful marker for differentiating benign from malignant lesions in the prostate, particularly when used in combination with markers of high molecular weight cytokeratins and the prostate-specific marker AMACR (P504S). p63 has also been shown to be a sensitive marker for lung squamous cell carcinomas (SqCC), with a sensitivity of ~90%. Specificity for lung SqCC, vs. lung adenocarcinoma (LADC), is approximately 80%. In breast tissue, p63 has been identified in myoepithelial cells of normal ducts.
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Immunogen
Recombinant partial human p53 protein was used as the immunogen for this p63 antibody.
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Storage
Store the p63 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Localization
Nuclear
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Short name
Anti-p63 / Tumor protein 63
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit IgG
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Alternative name
Antibodies to p63 / Tumor protein 63
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Alternative technique
antibodies
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Tissue
tumor
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MeSH Data
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Name
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Concept
Scope note:
A cytologic technique for measuring the functional capacity of tumor stem cells by assaying their activity. It is used primarily for the in vitro testing of antineoplastic agents.
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Tree numbers
- E01.370.225.500.383.910
- E01.370.225.500.388.930
- E05.200.500.383.910
- E05.200.500.388.930
- E05.242.383.910
- E05.242.417.500
- E05.337.550.200.800
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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