p62 Antibody / SQSTM1

• Catalog number
  R32218
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  p62 / SQSTM1
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P, IHC-F
• Recommended dilutions
  Western blot: 0.1-0.5ug/ml,IHC (Paraffin): 0.5-1ug/ml
• Notes
  Optimal dilution of the p62 antibody should be determined by the researcher.
• Intented use
  This p62 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q13501
• Purity
  Antigen affinity
• Description
  SQSTM1 (Sequestosome-1), also known as Ubiquitin-Binding Protein P62 or P62, is a protein that in humans is encoded by the SQSTM1 gene. The Src homology type 2 (SH2) domain is a highly conserved motif of about 100 amino acids which mediates protein-protein interactions by binding to phosphotyrosine.p56-lck, a T-cell-specific src family tyrosine kinase with an SH2 domain, is involved in T-cell signal transduction. The International Radiation Hybrid Mapping Consortium mapped the p62 gene to chromosome 5q35. Park et al. (1995) found that the p56-lck SH2 domain binds to p62 at the ser59 of p62 only when that serine is phosphorylated. Joung et al. (1996) expressed epitope-tagged p62 in Hela cells and showed that the expressed protein bound to the lck SH2 domain and that this binding was dependent on the N-terminal 50 amino acids of p62 but not on the tyrosine residue in this region.
• Immunogen
  Amino acids DEDGDLVAFSSDEELTMAMSYVKDDIFR of human p62 were used as the immunogen for the p62 antibody.
• Storage
  After reconstitution, the p62 antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Localization
  Cytoplasmic, membranous, nuclear
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  p62   SQSTM1  
• Gene symbol
  SQSTM1
• Short name
  Anti-p62 / SQSTM1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to p62 / SQSTM1
• Alternative technique
  antibodies
• Alternative to gene target
  sequestosome 1, A170 and OSIL and p60 and p62 and p62B and PDB3 and ZIP3, SQSTM1 and IDBG-61811 and ENSG00000161011 and 8878, K63-linked polyubiquitin binding, nuclei, Sqstm1 and IDBG-168358 and ENSMUSG00000015837 and 18412, BT.105440 and IDBG-640935 and ENSBTAG00000015591 and 338045

Gene info

• Identity
  HGNC:11280
• Gene
  SQSTM1
• Long gene name
  sequestosome 1
• Synonyms gene
  • PDB3
  • OSIL
• Synonyms gene name
  • Paget disease of bone 3
  • oxidative stress induced like
• Synonyms
  • p62
  • p60
  • p62B
  • A170
• Synonyms name
  • autophagy receptor p62
• GenBank acession
  • U46751
• Locus
  5q35.3
• Discovery year
  2000-06-13
• Entrez gene record
  8878
• Pubmed identfication
  • 8650207
  • 8551575
• Classification
  • Zinc fingers ZZ-type
• VEGA ID
  OTTHUMG00000150643

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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