NOXA1 Antibody

• Catalog number
  R31030
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  NOXA1
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the NOXA1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This NOXA1 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q86UR1
• Purity
  Antigen affinity
• Description
  NADPH oxidase activator 1, also called NOX Activator 1 or p51-NOX, is an enzyme that in humans is encoded by the NOXA1 gene. Hartz(2007) mapped the gene to chromosome 9q34.3 based on an alignment of the protein sequence with the genomic sequence(build 36.1). Banfi et al.(2003) mapped the mouse gene to chromosome 2. Using yeast 2-hybrid assays, Takeya et al.(2003) showed that the human protein interacted with constitutively active forms of RAC1 and RAC2. In vitro binding assays revealed that NOXA1 bound GTP-bound RAC1, but not GDP-bound RAC1. It also bound p47-PHOX (NCF1) and p41-NOX (NOXO1), and trp436 within the SH3 domain of NOXA1 was required for these interactions. Human cell lines or COS-7 cells cotransfected with NOXA1/p51-NOX and p41-NOX and either gp91-PHOX(CYBB) or NOX1 produced superoxide. Cells individually transfected with NOX1, p41-NOX, or p51-NOX and cells transfected with only p41-NOX and p51-NOX showed no superoxide production.
• Immunogen
  An amino acid sequence from the middle region of human NADPH oxidase activator 1 (RQVPRGEVFRPHRWHLKHLE) was used as the immunogen for this NOXA1 antibody.
• Storage
  After reconstitution, the NOXA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  NOXA1  
• Gene symbol
  NOXA1
• Short name
  Anti-NOXA1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to NOXA1
• Alternative technique
  antibodies
• Alternative to gene target
  NADPH oxidase activator 1, NY-CO-31 and p51NOX and SDCCAG31, NOXA1 and IDBG-93355 and ENSG00000188747 and 10811, Rac GTPase binding, Cytoplasm, Noxa1 and IDBG-144012 and ENSMUSG00000036805 and 241275, NOXA1 and IDBG-641039 and ENSBTAG00000012121 and 523157

Gene info

• Identity
  HGNC:10668
• Gene
  NOXA1
• Long gene name
  NADPH oxidase activator 1
• Synonyms gene
  • SDCCAG31
• Synonyms gene name
  • serologically defined colon cancer antigen 31
• Synonyms
  • NY-CO-31
  • FLJ25475
• GenBank acession
  • AF039697
• Locus
  9q34.3
• Discovery year
  1999-08-25
• Entrez gene record
  10811
• Pubmed identfication
  • 9610721
• VEGA ID
  OTTHUMG00000131781

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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