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Target antigen
NOXA1
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human, rat Predicted to work with: mouse
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Analyses
WB
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Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human NOXA1(176-195aa RQVPRGEVFRPHRWHLKHLE).
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the NOXA1 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The NOXA1 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
NOXA1(NADPH oxidase activator 1), also called NOX ACTIVATOR 1 or p51-NOX, is an enzyme that in humans is encoded by the NOXA1 gene. Hartz(2007) mapped the NOXA1 gene to chromosome 9q34.3 based on an alignment of the NOXA1 sequence with the genomic sequence(build 36.1). Banfi et al.(2003) mapped the mouse Noxa1 gene to chromosome 2. Using yeast 2-hybrid assays, Takeya et al.(2003) showed that human p51-NOX interacted with constitutively active forms of RAC1 and RAC2. In vitro binding assays revealed that p51-NOX bound GTP-bound RAC1, but not GDP-bound RAC1. p51-NOX also bound p47-PHOX(NCF1) and p41-NOX(NOXO1), and trp436 within the SH3 domain of p51-NOX was required for these interactions. Human cell lines or COS-7 cells cotransfected with p51-NOX and p41-NOX and either gp91-PHOX(CYBB) or NOX1 produced superoxide. Cells individually transfected with NOX1, p41-NOX, or p51-NOX and cells transfected with only p41-NOX and p51-NOX showed no superoxide production.
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Related articles
1. Banfi, B., Clark, R. A., Steger, K., Krause, K.-H. Two novel proteins activate superoxide generation by the NADPH oxidase NOX1. J. Biol. Chem. 278: 3510-3513, 2003. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 7/25/2007. 3. Takeya, R., Ueno, N., Kami, K., Taura, M., Kohjima, M., Izaki, T., Nunoi, H., Sumimoto, H. Novel human homologues of p47(phox) and p67(phox) participate in activation of superoxide-producing NADPH oxidases. J. Biol. Chem. 278: 25234-25246, 2003.
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Gene Name
NOXA1
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Protein Name
NADPH oxidase activator 1
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Gene Full Name
NADPH oxidase activator 1
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Synonyms
Antigen NY CO 31 antibody|Antigen NY-CO-31 antibody|FLJ25475 antibody|Inhibitory NADPH oxidase activator 1 antibody|MGC131800 antibody|NADPH oxidase activator 1 antibody|NCF2 like protein antibody|NCF2-like protein antibody|NOX activator 1 antibody|NOXA 1 antibody|Noxa1 antibody|NOXA1_HUMAN antibody|NY CO 31 antibody|p51 nox antibody|p51-nox antibody|p51NOX antibody|P67phox like factor antibody|P67phox-like factor antibody|SDCCAG31 antibody|Serologically defined colon cancer antigen 31 antibody
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Uniprot ID
Q86UR1
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
