MGMT Antibody

• Catalog number
  R30479
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  MGMT
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the MGMT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This MGMT antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P16455
• Purity
  Antigen affinity
• Description
  Methylated-DNA-protein-cysteine methytransferase(MGMT) is an enzyme that in humans is encoded by the MGMT gene. O(6)-alkylguanine is the major mutagenic and carcinogenic lesion in DNA induced by simple alkylating mutagens. MGMT gene is mapped to chromosome 10. Rydberg et al.(1990) concluded that the methyltransferase gene spans at least 15 kb. Gardner et al.(1991) refined the localization of the gene to 10q24.33-qter. Methylation of the gene's promoter plays a significant role in carcinogenesis.
• Immunogen
  An amino acid sequence from the middle region of human MGMT (PVFQQESFTRQVLWK) was used as the immunogen for this MGMT antibody.
• Storage
  After reconstitution, the MGMT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  MGMT  
• Gene symbol
  MGMT
• Short name
  Anti-MGMT
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to MGMT
• Alternative technique
  antibodies
• Alternative to gene target
  O-6-methylguanine-DNA methyltransferase, MGMT and IDBG-93188 and ENSG00000170430 and 4255, DNA-methyltransferase activity, nuclei, Mgmt and IDBG-211408 and ENSMUSG00000054612 and 17314

Gene info

• Identity
  HGNC:7059
• Gene
  MGMT
• Long gene name
  O-6-methylguanine-DNA methyltransferase
• Synonyms name
  • methylated-DNA--protein-cysteine methyltransferase
• GenBank acession
  • M29971
• Locus
  10q26.3
• Discovery year
  1989-10-16
• Entrez gene record
  4255
• RefSeq identity
  • NM_002412
• Classification
  • Methyltransferases
• VEGA ID
  OTTHUMG00000019261

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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