HSPB2 Antibody
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Catalog numberR32240
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PricePlease ask
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Size0.1mg
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CategoryAntibody
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Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
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FormAntigen affinity purified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenHSPB2
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB
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Recommended dilutionsWestern blot: 0.1-0.5ug/ml
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NotesOptimal dilution of the HSPB2 antibody should be determined by the researcher.
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Intented useThis HSPB2 antibodyis to be used only for research purposes and not for diagnostics..
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UniprotQ16082
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PurityAntigen affinity
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DescriptionAlpha-B-crystallin is a member of the alpha-crystallin/small heat-shock protein (HSP) family and under various neuropathologic conditions accumulates in reactive astrocytes and degenerating neurons. In the 5-prime flanking region of the CRYAB gene on 11q22-q23, where a constitutive DNaseI hypersensitive site is located, Iwaki et al. (1997) identified a gene transcribed in the opposite direction. Analysis of its mRNA structure by RT-PCR and 5-prime/3-prime RACE revealed that this gene is composed of 2 exons and encodes a new member of the alpha-crystallin/hsp family. They termed the gene HSPB2. Iwaki et al. (1997) also determined the complete genomic structure of the rat homolog of HSPB2. Southern blot analysis demonstrated that HSPB2 is expressed preferentially in skeletal muscle and heart but not in the lens, while the neighboring CRYAB gene is highly expressed in all 3 tissues. The 2 related genes are arranged in a head-to-head manner with an intergenic sequence of less than 1 kb, raising a possibility of shared regulatory elements for their expression.
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ImmunogenAmino acids 1-182 of human HSPB2 were used as the immunogen for the HSPB2 antibody.
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StorageAfter reconstitution, the HSPB2 antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolHSPB2-C11orf52, HSPB2
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Short nameAnti-HSPB2
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameAntibodies to HSPB2
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameHSPB2-C11orf52 readthrough (NMD candidate)
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Locus
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Discovery year2011-05-31
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameheat shock protein family B (small) member 2
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Synonyms gene name
- heat shock 27kD protein 2
- heat shock 27kDa protein 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-12-23
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Entrez gene record
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Pubmed identfication
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Classification
- Small heat shock proteins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
