Gli3 Antibody, Cy5.5 Conjugated
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Catalog numberbs-11565R-Cy5.5
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Price:
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Size0.1ml
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Long nameGli3 Polyclonal Antibody, Cy5.5 Conjugated
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CategoryConjugated Primary Antibodies
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ConjugationCy5.5
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Host OrganismRabbit (Oryctolagus cuniculus)
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Target AntigenGli3
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SpecificityThis is a highly specific antibody against Gli3.
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ModificationUnmodified
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Modification siteNone
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ClonalityPolyclonal
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ClonePolyclonal antibody
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Concentration1ug per 1ul
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SourceKLH conjugated synthetic peptide derived from human Gli3
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Tested applicationsIF(IHC-P)
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Recommended dilutionsIF(IHC-P)(1:50-200)
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CrossreactivityHuman, Mouse, Rat
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Crossreactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen backgroundIt has long been known that the overexpression of either Wnt-1 or the GLI proteins results in cancer; however, the molecular basis for this transformation was poorly understood. The Wnt-1 and GLI proteins have now been placed in a signaling cascade downstream of the mammalian homologs of the Drosophila hedgehog and patched proteins. The Drosophila segment polarity gene hedgehog (hh) encodes a secreted protein that appears to function in embryonic and imaginal disc patterning. The ptc gene, also identified as a Drosophila segment polarity gene, encodes the transmembrane protein patched, the expression of which is precisely regulated during embryonic development. Hedgehog has been shown to enhance the expression of the Wnt family of proteins through a signaling cascade involving the GLI transcription factors, while patched functions as a repressor opposing the effects of hedgehog. Mutations in the ptc gene, which result in unregulated hedgehog signaling, have been correlated with the most common type of cancer, basal cell carcinoma, which affects 750,000 individuals annually in the United States alone.
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Purification methodThis antibody was purified via Protein A.
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Storage conditionsKeep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission675nm/694nm
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SynonymsACLS; DNA binding protein; GCPS; Gli 3; GLI family zinc finger 3; GLI Kruppel family member GLI 3; GLI Kruppel family member GLI3 Greig cephalopolysyndactyly syndrome; GLI Kruppel family member GLI3; GLI3; GLI3 C-terminally truncated form; GLI3 form of 190 kDa; GLI3 form of 83 kDa; GLI3 full length protein; GLI3-190; GLI3-83; GLI3_HUMAN; GLI3FL; Glioma associated oncogene family zinc finger 3; Oncogene GLI3; PAP A; PAPA 1; PAPA; PAPA1; PAPB; PHS; PPD IV; PPDIV; Transcriptional activator GLI3; Transcriptional repressor GLI3R; Zinc finger protein GLI 3; Zinc finger protein GLI3.
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PropertiesIf you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor.
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Additional conjugationCy5.5, Cy5.5
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ConjugatedThese antibodies are excite for emission at 678nm and detected at a 694 nm wavelengths.
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French translationanticorps
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Gene target
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Short nameAnti-Gli3
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TechniqueAntibody, antibodies against human proteins, antibodies for, antibody Conjugates
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IsotypeImmunoglobulin G (IgG)
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LabelCy5.5
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Alternative nameAnti-Gli3 PAb Cy5.5
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Alternative techniqueantibodies
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Alternative to gene targetGLI family zinc finger 3, ACLS and GCPS and GLI3-190 and GLI3FL and PAP-A and PAPA and PAPA1 and PAPB and PHS and PPDIV, GLI3 and IDBG-13536 and ENSG00000106571 and 2737, metal ion binding, nuclei, Gli3 and IDBG-131686 and ENSMUSG00000021318 and 14634, GLI3 and IDBG-634502 and ENSBTAG00000010671 and 785371
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data