Emerin Antibody

• Catalog number
  R32593
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Emerin
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Notes
  Optimal dilution of the Emerin antibody should be determined by the researcher.
• Added buffer
  Lyophilized from 1X Phosphate Buffered Saline (PBS) with 2.5% BSA and 0.025% sodium azide
• Intented use
  This antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P50402
• Purity
  Antigen affinity
• Description
  Emerin is a serine-rich nuclear membrane protein that in humans is encoded by the EMD gene. And this gene is mapped to Xq28. Emerin is a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Emery–Dreifuss muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the EMD (also known clinically as STA) gene. Emerin appears to be involved in mechanotransduction, as emerin-deficient mouse fibroblasts failed to transduce normal mechanosensitive gene expression responses to strain stimuli. In cardiac muscle, emerin is also found complexed to beta-catenin at adherens junctions of intercalated discs, and cardiomyocytes from hearts lacking emerin showed beta-catenin redistribution as well as perturbed intercalated disc architecture and myocyte shape. This interaction appears to be regulated by glycogen synthase kinase 3 beta.
• Immunogen
  Amino acids 1-48 (MDNYADLSDTELTTLLRRYNIPHGPVVGSTRRLYEKKIFEYETQRRRL-human) were used as the immunogen for the Emerin antibody.
• Storage
  After reconstitution, the Emerin antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Localization
  Nuclear membrane, nuclear
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Emerin  
• Gene symbol
  EMD, LEMD2
• Short name
  Anti- Emerin
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to Emerin
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:3331
• Gene
  EMD
• Long gene name
  emerin
• Synonyms gene name
  • Emery-Dreifuss muscular dystrophy
• Synonyms
  • STA
  • LEMD5
• Synonyms name
  • LEM domain containing 5
• GenBank acession
  • X82434
• Locus
  Xq28
• Discovery year
  2001-06-22
• Entrez gene record
  2010
• Classification
  • LEM domain containing
• VEGA ID
  OTTHUMG00000033186
• Locus Specific Databases
  • Leiden Muscular Dystrophy Pages
  • UMD Locus Specific Databases
  • Leiden Muscular Dystrophy pages
  • Global Variome shared LOVD
  • LRG_745

Gene info

• Identity
  HGNC:21244
• Gene
  LEMD2
• Long gene name
  LEM domain nuclear envelope protein 2
• Synonyms gene name
  • LEM domain containing 2
• Synonyms
  • dJ482C21.1
  • NET25
  • LEM2
• Synonyms name
  • lamina-associated polypeptide-emerin-MAN1 domain containing 2
• Locus
  6p21.31
• Discovery year
  2003-05-29
• Entrez gene record
  221496
• Pubmed identfication
  • 16339967
  • 28056360
  • 28242692
  • 31147383
• RefSeq identity
  • NM_181336
• Classification
  • LEM domain containing
• VEGA ID
  OTTHUMG00000014535

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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