EMAP II Antibody
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Catalog numberN1155-25UG
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PricePlease ask
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Size0.025mg
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CategoryAntibody
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Concentration0.5 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
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FormPurified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenEMAP II
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB
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Recommended dilutionsWestern blot: 2-5ug/ml
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NotesProvided assay concentrations are suggestions only, antibody titration may be required for optimal results.
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Added buffer1X Phosphate Buffered Saline (PBS), pH 7.4
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Intented useThis EMAP II antibodyis to be used only for research purposes and not for diagnostics..
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Gene ID9255
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PurityThis antibody was purified by Protein A method
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DescriptionEMAP II (Endothelial monocyte-activating polypeptide 2) is a pro-inflammatory cytokine and participant in apoptotic cell death. It is synthesized as a precursor (pro-EMAP II), lacking a secretion signal peptide. In apoptotic cells, the ~34 kDa pro-form of EMAP II is cleaved by Caspase-7 (and Caspase-3 to a lesser extent) at aspartate 144 to its mature, ~20 kDa form. The mature form of EMAP II has been found to have many different functions. In vivo, it recruits leukocytes to the site of apoptotic cell death. In vitro it has been shown to activate endothelial cells (and the release of von Willebrand factor, E-selectin and P-selectin), neutrophils, and induces macrophage expression of TNFa and TF (tissue factor). EMAP II is also a possible biomarker for brain injury, having been found to be differentially expressed in two types: traumatic injury shows an increase and ischemic injury shows a decrease in EMAP II expression (Dave, et al, 2014).
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ImmunogenA recombinant protein fragment from the C-terminal region of human EMAP II was used as the immunogen for this antibody.
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StorageAliquot the antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolEML1, EML2, EML5, AIMP1
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Short nameAnti-EMAP II
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameAntibodies to EMAP II
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameEMAP like 1
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Synonyms gene
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Synonyms gene name
- echinoderm microtubule associated protein like 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-07-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- WD repeat domain containing
- EMAP like
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameEMAP like 2
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Synonyms gene name
- echinoderm microtubule associated protein like 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2002-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- WD repeat domain containing
- EMAP like
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameEMAP like 5
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Synonyms gene name
- echinoderm microtubule associated protein like 5
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-12-17
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Entrez gene record
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Classification
- WD repeat domain containing
- EMAP like
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameaminoacyl tRNA synthetase complex interacting multifunctional protein 1
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Synonyms gene
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Synonyms gene name
- small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-04-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data