EMAP II Antibody

• Catalog number
  N1155-100UG
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Form
  Purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  EMAP II
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 2-5ug/ml
• Notes
  Provided assay concentrations are suggestions only, antibody titration may be required for optimal results.
• Added buffer
  1X Phosphate Buffered Saline (PBS), pH 7.4
• Intented use
  This EMAP II antibodyis to be used only for research purposes and not for diagnostics..
• Gene ID
  9255
• Purity
  This antibody was purified by Protein A method
• Description
  EMAP II (Endothelial monocyte-activating polypeptide 2) is a pro-inflammatory cytokine and participant in apoptotic cell death. It is synthesized as a precursor (pro-EMAP II), lacking a secretion signal peptide. In apoptotic cells, the ~34 kDa pro-form of EMAP II is cleaved by Caspase-7 (and Caspase-3 to a lesser extent) at aspartate 144 to its mature, ~20 kDa form. The mature form of EMAP II has been found to have many different functions. In vivo, it recruits leukocytes to the site of apoptotic cell death. In vitro it has been shown to activate endothelial cells (and the release of von Willebrand factor, E-selectin and P-selectin), neutrophils, and induces macrophage expression of TNFa and TF (tissue factor). EMAP II is also a possible biomarker for brain injury, having been found to be differentially expressed in two types: traumatic injury shows an increase and ischemic injury shows a decrease in EMAP II expression (Dave, et al, 2014).
• Immunogen
  A recombinant protein fragment from the C-terminal region of human EMAP II was used as the immunogen for this antibody.
• Storage
  Aliquot the antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  EMAP  
• Gene symbol
  EML1, EML2, EML5, AIMP1
• Short name
  Anti-EMAP II
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to EMAP II
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:3330
• Gene
  EML1
• Long gene name
  EMAP like 1
• Synonyms gene
  • EMAPL
• Synonyms gene name
  • echinoderm microtubule associated protein like 1
• Synonyms
  • EMAP
  • HuEMAP
  • ELP79
• GenBank acession
  • AK023861
• Locus
  14q32.2
• Discovery year
  1997-07-11
• Entrez gene record
  2009
• Pubmed identfication
  • 9226380
  • 10521658
• RefSeq identity
  • NM_001008707
• Classification
  • WD repeat domain containing
  • EMAP like
• VEGA ID
  OTTHUMG00000171529

Gene info

• Identity
  HGNC:18035
• Gene
  EML2
• Long gene name
  EMAP like 2
• Synonyms gene name
  • echinoderm microtubule associated protein like 2
• Synonyms
  • EMAP2
  • ELP70
  • EMAP-2
• Synonyms name
  • echinoderm MT-associated protein (EMAP)-like protein 70
  • microtubule-associated protein like echinoderm EMAP
• GenBank acession
  • AF103939
• Locus
  19q13.32
• Discovery year
  2002-02-15
• Entrez gene record
  24139
• Pubmed identfication
  • 11694528
  • 10521658
• RefSeq identity
  • NM_012155
• Classification
  • WD repeat domain containing
  • EMAP like
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000182127

Gene info

• Identity
  HGNC:18197
• Gene
  EML5
• Long gene name
  EMAP like 5
• Synonyms gene name
  • echinoderm microtubule associated protein like 5
• Synonyms
  • HuEMAP-2
  • EMAP-2
  • FAP16
• GenBank acession
  • AY357725
• Locus
  14q31.3
• Discovery year
  2002-12-17
• Entrez gene record
  161436
• Classification
  • WD repeat domain containing
  • EMAP like
• VEGA ID
  OTTHUMG00000170811

Gene info

• Identity
  HGNC:10648
• Gene
  AIMP1
• Long gene name
  aminoacyl tRNA synthetase complex interacting multifunctional protein 1
• Synonyms gene
  • SCYE1
• Synonyms gene name
  • small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating)
• Synonyms
  • EMAPII
  • EMAP-2
  • p43
• Synonyms name
  • EMAP II
  • ARS-interacting multifunctional protein 1
• GenBank acession
  • U10117
• Locus
  4q24
• Discovery year
  2000-04-13
• Entrez gene record
  9255
• Pubmed identfication
  • 7929199
  • 7545917
• RefSeq identity
  • NM_004757
• VEGA ID
  OTTHUMG00000131217

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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