E2F3 Antibody

• Catalog number
  R32617
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  E2F3
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  Optimal dilution of the E2F3 antibody should be determined by the researcher.
• Added buffer
  Lyophilized from 1X Phosphate Buffered Saline (PBS) with 2.5% BSA and 0.025% sodium azide
• Intented use
  This antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  O00716
• Purity
  Antigen affinity
• Description
  Transcription factor E2F3, also known as KIAA0075, is a protein that in humans is encoded by the E2F3 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. By fluorescence in situ hybridization, E2F3 gene is mapped to 6q22.3. The induction of specific E2F activities is an essential component in the MYC pathways that control cell proliferation and cell fate decisions. This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle.
• Immunogen
  Amino acids 278-320 (QSCTLDLKLLTEDSENQRLAYVTYQDIRKISGLKDQTVIVVKA-human) were used as the immunogen for the E2F3 antibody.
• Storage
  After reconstitution, the E2F3 antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  E2F3  
• Gene symbol
  E2F3, E2F3-IT1
• Short name
  Anti- E2F3
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to E2F3
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:3115
• Gene
  E2F3
• Long gene name
  E2F transcription factor 3
• GenBank acession
  • Y10479
• Locus
  6p22.3
• Discovery year
  1994-08-31
• Entrez gene record
  1871
• Pubmed identfication
  • 8246996
• Classification
  • E2F transcription factors
• VEGA ID
  OTTHUMG00000016389

Gene info

• Identity
  HGNC:41329
• Gene
  E2F3-IT1
• Long gene name
  E2F3 intronic transcript 1
• Synonyms gene name
  • E2F3 intronic transcript 1 (non-protein coding)
• Locus
  6p22.3
• Discovery year
  2011-05-24
• Classification
  • Intronic transcripts
• VEGA ID
  OTTHUMG00000014337

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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