Cdk4 Antibody
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Catalog numberF52368-0.4ML
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PricePlease ask
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Size0.4 ml
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CategoryAntibody
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ConcentrationIn 1X PBS, pH 7.4, with 0.09% sodium azide
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FormAntigen affinity purified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenCdk4
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsIHC, IF, ELISA
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Recommended dilutionsIHC (Paraffin): 1:25,Immunofluorescence: 1:25
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NotesTitration of the Cdk4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis Cdk4 antibodyis to be used only for research purposes and not for diagnostics..
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UniprotP35426
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PurityAntigen affinity
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DescriptionSer/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).
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ImmunogenA portion of amino acids 272-303 from the rat protein was used as the immunogen for this Cdk4 antibody.
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StorageAliquot the Cdk4 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolCDK4, CDKN2C, CDKN2D
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Short nameAnti-Cdk4
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit Ig
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Alternative nameAntibodies to Cdk4
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Alternative techniqueantibodies
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Alternative to gene targetcyclin-dependent kinase 4, CMM3 and PSK-J3, CDK4 and IDBG-43642 and ENSG00000135446 and 1019, transferase activity, nuclei, Cdk4 and IDBG-194056 and ENSMUSG00000006728 and 102641469,12567
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Gene info
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Identity
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Gene
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Long gene namecyclin dependent kinase 4
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-07-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Cyclin dependent kinases
- MicroRNA protein coding host genes
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namecyclin dependent kinase inhibitor 2C
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Synonyms gene name
- cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4)
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Synonyms
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GenBank acession
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Locus
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Discovery year1995-07-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ankyrin repeat domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecyclin dependent kinase inhibitor 2D
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Synonyms gene name
- cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4)
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Synonyms
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Locus
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Discovery year1995-07-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ankyrin repeat domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data