Anti-MDM2 Antibody

• Catalog number
  F42277-0.4ML
• Price
  Please ask
• Size
  0.4 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Anti-MDM2
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC, ELISA
• Recommended dilutions
  Western blot: 1:1000,IHC (Paraffin): 1:10-1:50
• Notes
  Titration of the anti-MDM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This anti-MDM2 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q00987
• Purity
  Purified
• Description
  MDM2 is a target of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle,apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.
• Immunogen
  A portion of amino acids 393-424 from the human protein was used as the immunogen for this anti-MDM2 antibody.
• Storage
  Aliquot the anti-MDM2 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  MDM2  
• Gene symbol
  MDM2
• Short name
  Anti-Anti-MDM2
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to Anti-MDM2
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:6973
• Gene
  MDM2
• Long gene name
  MDM2 proto-oncogene
• Synonyms gene name
  • mouse double minute 2, human homolog of; p53-binding protein
  • Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse)
  • Mdm2 p53 binding protein homolog (mouse)
  • MDM2 proto-oncogene, E3 ubiquitin protein ligase
• Synonyms
  • HDM2
  • MGC5370
• Locus
  12q15
• Discovery year
  1993-12-10
• Entrez gene record
  4193
• Pubmed identfication
  • 1614537
  • 16905769
  • 29123033
• RefSeq identity
  • NM_002392
• Classification
  • Zinc fingers RANBP2-type
  • Ring finger proteins
• VEGA ID
  OTTHUMG00000142827

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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