Stem Cell Factor, Recombinant, Human (SCF, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF)

  • Catalog number
    MBS651070
  • Price
    Please ask
  • Size
    5x0.01 mg
  • Products_type
    Active Protein
  • Products_short_name
    [Stem Cell Factor]
  • Other_names
    [stem cell factor]
  • Purity
    Highly Purified95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. Endotoxin: 0.1ng/ug (IEU/ug).
  • Form
    Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with sterile ddH2O,0.1% HSA or BSA to 0.1mg/ml, which can then be further diluted with in other aqueous solutions.
  • Storage_stability
    Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O,0.1% HSA or BSA. Aliquot and store at -20 degree C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
  • Description
    For cells, cell lines and tissues in culture till half confluency. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Test
    Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
  • Gene target
    Stem   Factor   SCF   CD117   c-kit   Steel   Factor   Mast   Growth   Factor   MGF  
  • Gene symbol
    KITLG, STAT5A, KIT
  • Short name
    Stem Factor, Recombinant, (SCF, CD117 c-kit, Steel Factor, Mast Growth Factor, MGF)
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Host
    Sf9 insect cells
  • Species
    Human, Humans
  • Alternative name
    progenitor cellular Factor, Rec., H. sapiens (SCF, CD117 c-reagent, Steel Factor, Mast cellular Growth Factor, MGF)
  • Alternative technique
    rec, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
  • Tissue
    cell, stem
Gene info
Gene info
Gene info
MeSH Data
  • Name
    Primary Cell Culture
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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Stem Factor Recombinant SCF CD117 c kit Steel Factor Mast Gr
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