-
Products_type
Antibody
-
Products_short_name
[CD117]
-
Products_name_syn
[Anti -CD117, phosphorylated (Tyr719) (c-kit, Stem Cell Factor, SCF Receptor, Steel Factor Receptor, Mast Cell Growth Factor, MGF)]
-
Other_names
[chromogranin-A preproprotein; Chromogranin-A; chromogranin-A; SP-I; pituitary secretory protein I; parathyroid secretory protein 1; betagranin (N-terminal fragment of chromogranin A); chromogranin A (parathyroid secretory protein 1); Pituitary secretory protein I; SP-ICleaved into the following 13 chains:Vasostatin-1; Alternative name(s):; Vasostatin IVasostatin-2; Alternative name(s):; Vasostatin II]
-
Other_gene_names
[CHGA; CHGA; CGA; CgA; SP-I]
-
Clonality
Polyclonal
-
Reactivity
Human, Mouse
-
Specificity
Recognizes endogenous levels of c-Kit protein only when phosphorylated at Tyr719. This antibody crossreacts with tyrosine-phosphorylated PDGF receptor but does not crossreact with other tyrosine-phosphorylated family members. Species Crossreactivity
-
Purity
Affinity Purified Purified by protein A and peptide affinity chromatography.
-
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
-
Storage_stability
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
-
Tested_application
Western Blot (WB)
-
Description
For cells, cell lines and tissues in culture till half confluency. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
-
Test
Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
-
Gene target
-
Gene symbol
KITLG, STAT5A, KIT
-
Short name
CD117, phosphorylated (Tyr719) (c-kit, Stem Factor, SCF Receptor, Steel Factor Receptor, Mast Growth Factor, MGF)
-
Host
Rabbit
-
Isotype
IgG
-
Alternative name
CD117, phosphorylated (Tyr719) (c-reagent, progenitor cellular Factor, SCF Receptor, Steel Factor Receptor, Mast cellular Growth Factor, MGF)
-
Alternative technique
kits
-
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
-
Tissue
cell, stem
-
