Reverse Transcriptase, M-MLV (M-MLV-RT) Recombinant

Products_type

Recombinant Protein
Products_short_name

[Reverse Transcriptase, M-MLV]
Purity

Molecular Biology Grade90%, Verified by SDS-PAGE
Form

Supplied as a liquid in 20mM Tris HCl, pH 7.5, 200mM sodium chloride, 0.1mM EDTA, 1mM DTT, 0.01% NP-40 and 50% glycerol.
Storage_stability

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source

Recombinants or rec. proteins
Group

recombinants
Description

Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
Goup

reverse transcription

Gene target

Transcriptase M-MLV M-MLV-RT
Gene symbol

CPT1B, IRAK3, PGAM2, CYP20A1, USP16, CYB5B, H3C14, ABCB10, NEFM, MPRIP
Short name

Reverse Transcriptase, M-MLV (M-MLV-RT) Recombinant
Technique

Recombinant, reverse, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Host

Recombinant expressed in E Coli
Alternative name

Reverse Transcriptase, M-MLV (M-MLV-RT) Rec.
Alternative technique

rec, transctription

Identity

HGNC:20503
Gene

H3C14
Long gene name

H3 clustered histone 14
Synonyms gene
- H3F2
- H3FM
- HIST2H3C
Synonyms gene name
- H3 histone family, member M
- histone 2, H3c
- histone cluster 2, H3c
- histone cluster 2 H3 family member c
Synonyms
- MGC9629
- H3/m
- H3
- H3.2
- H3/M
GenBank acession
- AF531307
Locus

1q21.2
Discovery year

2003-02-19
Entrez gene record

126961
Pubmed identfication
- 12408966
- 3013246
RefSeq identity
- NM_021059
Classification
- H3 histones
VEGA ID

OTTHUMG00000012190

Identity

HGNC:7734
Gene

NEFM
Long gene name

neurofilament medium chain
Synonyms gene
- NEF3
Synonyms gene name
- neurofilament, medium polypeptide 150kDa
- neurofilament, medium polypeptide
- neurofilament medium
Synonyms
- NFM
- NF-M
GenBank acession
- BC002421
Locus

8p21.2
Discovery year

1989-10-18
Entrez gene record

4741
Pubmed identfication
- 1348579
RefSeq identity
- NM_005382
Classification
- Intermediate filaments Type IV
VEGA ID

OTTHUMG00000131990

Name

Reverse Transcriptase Polymerase Chain Reaction
Concept

Scope note: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Tree numbers
- E05.393.620.500.725
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data