Reverse Transcriptase, M-MLV (M-MLV-RT) Recombinant
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Catalog numberMBS636119
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Price897.14 USD
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Size50K Units
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Products_typeRecombinant Protein
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Products_short_name[Reverse Transcriptase, M-MLV]
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PurityMolecular Biology Grade90%, Verified by SDS-PAGE
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FormSupplied as a liquid in 20mM Tris HCl, pH 7.5, 200mM sodium chloride, 0.1mM EDTA, 1mM DTT, 0.01% NP-40 and 50% glycerol.
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Storage_stabilityMay be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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DescriptionReverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
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Goupreverse transcription
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Gene target
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Gene symbolCPT1B, IRAK3, PGAM2, CYP20A1, USP16, CYB5B, H3C14, ABCB10, NEFM, CSF1
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Short nameReverse Transcriptase, M-MLV (M-MLV-RT) Recombinant
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TechniqueRecombinant, reverse, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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HostRecombinant expressed in E Coli
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Alternative nameReverse Transcriptase, M-MLV (M-MLV-RT) Rec.
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Alternative techniquerec, transctription
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Gene info
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Identity
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Gene
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Long gene namecarnitine palmitoyltransferase 1B
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Synonyms gene name
- carnitine palmitoyltransferase 1B (muscle)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1996-08-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameinterleukin 1 receptor associated kinase 3
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Synonyms gene name
- interleukin-1 receptor-associated kinase 3
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-07-17
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Entrez gene record
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Pubmed identfication
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Classification
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namephosphoglycerate mutase 2
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Synonyms gene name
- phosphoglycerate mutase 2 (muscle)
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Synonyms
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Locus
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Discovery year1989-06-30
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Entrez gene record
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Classification
- Phosphoglycerate mutases
- Bisphosphoglycerate phosphatases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecytochrome P450 family 20 subfamily A member 1
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Synonyms gene name
- cytochrome P450, family 20, subfamily A, polypeptide 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-03-20
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Entrez gene record
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RefSeq identity
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Classification
- Cytochrome P450 family 20
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameubiquitin specific peptidase 16
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Synonyms gene name
- ubiquitin specific protease 16
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-07-09
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Pubmed identfication
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Classification
- Ubiquitin specific peptidases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecytochrome b5 type B
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Synonyms gene name
- cytochrome b5 type B (outer mitochondrial membrane)
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Synonyms
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Locus
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Discovery year2006-01-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameH3 clustered histone 14
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Synonyms gene
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Synonyms gene name
- H3 histone family, member M
- histone 2, H3c
- histone cluster 2, H3c
- histone cluster 2 H3 family member c
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-02-19
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- H3 histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameATP binding cassette subfamily B member 10
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Synonyms gene name
- ATP-binding cassette, sub-family B (MDR/TAP), member 10
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-10-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ATP binding cassette subfamily B
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameneurofilament medium chain
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Synonyms gene
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Synonyms gene name
- neurofilament, medium polypeptide 150kDa
- neurofilament, medium polypeptide
- neurofilament medium
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Synonyms
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GenBank acession
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Locus
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Discovery year1989-10-18
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Intermediate filaments Type IV
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecolony stimulating factor 1
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Synonyms gene name
- colony stimulating factor 1 (macrophage)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
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Tree numbers
- E05.393.620.500.725
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data