Recombinant MLV Reverse Transcriptase

Short description

Leukemia Virus reverse transcriptase, recombinant protein from E. coli.
Source

Recombinants or rec. proteins
Group

recombinants
Description

Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
Goup

reverse transcription

Gene target

MLV Transcriptase
Short name

Recombinant MLV Reverse Transcriptase
Technique

Recombinant, reverse, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Creative Biolabs advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Alternative name

Rec. MLV Reverse Transcriptase
Alternative technique

rec, transctription

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data