Rat Anti-Mouse CD62L/L-selectin-LE/AF
-
Catalog numberMBS673422
-
PricePlease ask
-
Size5x0.5 mg
-
-
Products_typeAntibody
-
Products_short_name[CD62L (L-Selectin)]
-
Products_name_syn[Rat Anti-Mouse CD62L/L-selectin - Low Endotoxin/Azide-Free; Rat Anti-Mouse CD62L (L-Selectin)]
-
ClonalityMonoclonal
-
Clone[MEL-14]
-
ReactivityMouse
-
SpecificityCD62L/L-selectin, Mr 90 kDa CD62L, also known as L-selectin, is a type I transmembrane glycoprotein with lectin-like and Epidermal Growth Factor-like domains.^1, 2 It binds to sialylated oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes.^1, 2 In the mouse, CD62L is expressed on most thymocytes and on peripheral leukocytes, including B and T lymphocytes, neutrophils, monocytes and eosinophils.^3-7 This member of the selectin family of cell adhesion molecules appears to be required for lymphocyte homing to peripheral lymph nodes and for leukocyte extravasation at sites of inflammation.^1, 2, 7-9 Expression of CD62L on lymphocytes and neutrophils is rapidly down-regulated upon cell activation and the level of CD62L expression, along with other markers, distinguishes naive T cells from effector/memory T cells.^10-13 The monoclonal antibody MEL-14 blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling.^1, 2, 7-9
-
FormLow Endotoxin, Azide - Free (LE/AF)
-
Storage_stabilityThe purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The Spectral Red (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), and allophycocyanin-Cyanine 7 (APCCY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
-
Tested_applicationFlow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin nameMus musculus, Rattus norvegicus
-
AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
-
Gene target
-
Gene symbolFUT3, HPS4, NPAS4, SELL
-
Short nameAnti-Mouse CD62L/L-selectin-LE/AF
-
Techniqueanti-, Mouse, anti, antibody to, antibodies, mouses
-
HostRat
-
IsotypeRat (Fisher) IgG2a kappa
-
SpeciesMouse, Mouses, Rats
-
Alternative nameRat Antibody toMouse CD62L/L-selectin-LE/AF
-
Alternative techniquemurine, antibodies
-
Gene info
-
Identity
-
Gene
-
Long gene namefucosyltransferase 3 (Lewis blood group)
-
Synonyms gene
-
Synonyms gene name
- fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group included)
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year1993-04-08
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Fucosyltransferases
- Blood group antigens
- CD molecules
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene nameHPS4 biogenesis of lysosomal organelles complex 3 subunit 2
-
Synonyms gene name
- Hermansky-Pudlak syndrome 4
-
Synonyms
-
Locus
-
Discovery year2001-06-28
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Biogenesis of lysosomal organelles complex 3
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene nameneuronal PAS domain protein 4
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2005-09-26
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Basic helix-loop-helix proteins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameselectin L
-
Synonyms gene
-
Synonyms gene name
- lymphocyte adhesion molecule 1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1989-06-07
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Sushi domain containing
- Selectins
- C-type lectin domain containing
- CD molecules
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
