Rat Anti Mouse CD62L Antibody-FITC

• Catalog number
  MO62LF(V25)
• Price
  Please ask
• Size
  0,025 mg

• Description
  Rat Anti Mouse CD62L Antibody-FITC is a mouse monoclonal antibody, specific for [MEL-14] clones, this antibody was conjugated with FITC format and reported for use in flow cytometric analysis to identify CD62L glycoproteins in B, Nk and T cells subsets, and also in granulocytes and monocytes, CD62L actsas a homing factor of lymphocytes to high endothelial venules HEV and rolling on activated endothelium.
• Other rodent antibodies
• Clone
  MEL-14
• Conjugate
  FITC
• Gene Name
  CD62L
• Other Names of gene
  L-selectin, LECAM-1, Ly-22, LAM-1, MEL-14
• Concentration
  500 µg/mL
• Application
  Flow Cytometry Method
• Immunogen
  C3H/eb mouse B lymph oma 38C-17
• Formulation
  Aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3).
• Storage
  2-8°C, store in dark, do not freeze
• Expiration
  After 1 year
• Remark
  For Recherche Use Only.
• Additional description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
• Properties
  If you buy Antibodies supplied by Immunostep they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Immunostep Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Immunostep FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• French translation
  anticorps

• Gene target
  CD62L  
• Gene symbol
  SELL
• Short name
  Anti Mouse CD62L Antibody-FITC
• Technique
  anti mouse, Antibody, Mouse, anti, FITC, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, Fluorescein, mouses
• Host
  Rat
• Isotype
  Rat IgG2a
• Label
  FITC
• Species
  Mouse, Mouses, Rats
• Alternative name
  Rat CD62L Mouse Monoclonal Antibody [MEL-14]-FITC
• Alternative technique
  antibodies, murine, fluorescine

Gene info

• Identity
  HGNC:10720
• Gene
  SELL
• Long gene name
  selectin L
• Synonyms gene
  • LYAM1
  • LNHR
• Synonyms gene name
  • lymphocyte adhesion molecule 1
• Synonyms
  • LSEL
  • LAM1
  • LAM-1
  • hLHRc
  • Leu-8
  • Lyam-1
  • PLNHR
  • CD62L
• GenBank acession
  • M25280
• Locus
  1q24.2
• Discovery year
  1989-06-07
• Entrez gene record
  6402
• Pubmed identfication
  • 2664786
  • 1375831
• RefSeq identity
  • NM_000655
• Classification
  • Sushi domain containing
  • Selectins
  • C-type lectin domain containing
  • CD molecules
• VEGA ID
  OTTHUMG00000034809

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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