Rabbit Polyclonal (IgG) to Sheep YWHAE / 14-3-3 Epsilon
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Catalog number
MBS244175
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Price
Please ask
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Size
0.05 mL
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Products_type
Antibody
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Products_short_name
[YWHAE / 14-3-3 Epsilon]
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Products_name_syn
[Anti-YWHAE / 14-3-3 Epsilon Antibody (N-Terminus) IHC-plus; YWHAE; 14-3-3E; 14-3-3 epsilon; 14-3-3 protein epsilon; MDCR; MDS; KCIP-1; Sheep YWHAE; 14-3-3 Epsilon]
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Other_names
[14-3-3 protein epsilon; 14-3-3 protein epsilon; 14-3-3 protein epsilon; 14-3-3 epsilon; epididymis luminal protein 2; protein kinase C inhibitor protein-1; mitochondrial import stimulation factor L subunit; tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon]
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Products_gene_name
[YWHAE]
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Other_gene_names
[YWHAE; YWHAE; MDS; HEL2; MDCR; KCIP-1; 14-3-3E; 14-3-3E]
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Clonality
Polyclonal
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Reactivity
Bovine, Chicken, Human, Mouse, Rabbit, Rat, Sheep Predicted Reactivity: Mammal (at least 90% immunogen sequence identity)
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Specificity
Recognizes the acetylated N-terminal of 14-3-3 epsilon. It is a member of the 14-3-3 family which consists of 30 kD proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 epsilon is expressed in all mammals. It is deleted in small cell lung cancers disrupting the G(2) checkpoint. During apoptosis, cleavage of 14-3-3 protein by caspase-3 increases the affinity between bad and Bcl-x(L). When bound by ERK, 14-3-3 epsilon is thought to deactivate heat shock factor 1 in cells recovering from heat shock. It is also a potential suicide susceptibility gene. May not react with recombinant proteins that are not N-acetylated.
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Form
Serum, 0.09% sodium azide.
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Storage_stability
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.
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Tested_application
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Group
Polyclonals and antibodies
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
YWHAQ, YWHAG, YWHAZ, MIR1302-3, MIR509-3, MIR7-3, PIRC14, PIRC12
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Short name
Rabbit Polyclonal (IgG) YWHAE / 14-3-3 Epsilon
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Technique
Polyclonal, Rabbit, IgG, IgGs, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG, IgG
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Species
Sheep, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
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Alternative name
production species: rabbit polyclonal (Immunoglobulin G) to ovine tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide / 14-3-3 Epsilon
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Alternative technique
polyclonals, rabbit-anti, immunoglobulins
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Alternative to gene target
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide, 14-3-3E and HEL2 and KCIP-1 and MDCR and MDS, YWHAE and IDBG-14629 and ENSG00000108953 and 7531, phosphoprotein binding, Plasma membranes, Ywhae and IDBG-200597 and ENSMUSG00000020849 and 22627, YWHAE and IDBG-632682 and ENSBTAG00000005664 and 282125
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Gene info
Gene info
Gene info
Gene info
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Identity
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Gene
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Long gene name
microRNA 1302-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
Gene info
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Identity
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Gene
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Long gene name
microRNA 509-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2007-10-24
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Entrez gene record
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RefSeq identity
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Classification
Gene info
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Identity
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Gene
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Long gene name
microRNA 7-3
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
Gene info
Gene info
Product images
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