Rabbit F(ab')2 Anti-Mouse IgG(H+L)-BIOT
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Catalog number
MBS675134
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Price
Please ask
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Size
5x0.5 mg
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Products_type
Secondary Antibody
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Products_short_name
[Rabbit F(ab')2 Anti-Mouse IgG(H+L)]
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Clonality
Polyclonal
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Reactivity
Mouse
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Specificity
Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b and IgG3, and with the light chains of mouse IgM and IgA as demonstrated by ELISA or FLISA
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Purity
Affinity chromatography on mouse IgG covalently coupled to agarose. Gel filtration chromatography of pepsin digested antibody
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Form
Biotin (BIOT) Conjugate
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Storage_stability
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
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Tested_application
Immunofluorescence (IF), ELISA (EIA), FLISA, Western Blot (WB), Immunoblot (IB), Immunohistochemistry (IHC)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
f(ab')2s
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Oryctolagus cuniculus
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Gene target
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Short name
Rabbit F(ab')2 Anti-Mouse IgG(H+L)-BIOT
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Technique
F(ab')2, Rabbit, anti-, Mouse, anti, IgG, antibody to, antibodies, F(ab')2 antibody fragments are the antigen binding variable regions obtained by enzymatic cutting of the parent IgG at neutral ph. IgGs, mouses
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Host
Pepsin digest of rabbit anti-mouse IgG (H+L). Rabbits
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Isotype
IgG, F(ab')2 Fragment Affinity-Purified Secondary Antibodies, IgG
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Species
Mouse, Mouses
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Alternative name
production species: rabbit F(Antibody')2 Antibody toMouse Immunoglobulin G(heavy + light chain)-BIOT
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Alternative technique
secondairy, rabbit-anti, murine, antibodies, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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