Rabbit Anti-Rat IgG (H+L)-BIOT
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Catalog numberMBS674913
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Price:
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Size5x1 mg
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Products_typeSecondary Antibody
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Products_short_name[Rabbit Anti-Rat IgG (H+L)]
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Products_name_syn[Rabbit Anti-Rat IgG(H+L) - Biotin; Rabbit Anti-Rat IgG (H+L)]
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ClonalityPolyclonal
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ReactivityRat
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SpecificityReacts with the heavy and light chains of rat IgG1, IgG2a, IgG2b and IgG2c, and with the light chains of rat IgM as demonstrated by ELISA and/or flow cytometry.
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PurityAffinity chromatography on pooled rat IgG covalently linked to agarose.
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FormBiotin (BIOT) Conjugate
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Storage_stabilityThe purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. The fluorescein (FITC) conjugate is supplied as 1.0 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C. The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C. The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2-8 degree C. Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
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Tested_applicationELISA; Immunoblot; Immunofluorescence; Immunohistochemistry; Western Blot
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameOryctolagus cuniculus, Rattus norvegicus
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Gene target
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Short nameRabbit Anti- IgG (H+L)-BIOT
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TechniqueRabbit, anti-, anti, IgG, antibody to, antibodies, IgGs
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HostRabbit, Rabbits
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IsotypeIgG, IgG
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SpeciesRat, Rats
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Alternative nameproduction species: rabbit Antibody toRat Immunoglobulin G (heavy + light chain)-BIOT
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Alternative techniquerabbit-anti, antibodies, immunoglobulins
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data