Rabbit anti Sheep IgM (Fc specific), conjugated with Biotin
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Catalog number
MBS571718
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Price
Please ask
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Size
1 mL
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Products_type
Secondary Antibody
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Products_short_name
[Rabbit anti Sheep IgM (Fc specific)]
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Clonality
Polyclonal
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Reactivity
Sheep
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Specificity
Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to Sheep IgM, Fc specific.
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Purity
Hyperimmune antisera with strong precipitating activity are selected for purification of the IgG fraction by Protein G chromatography.
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Storage_stability
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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Tested_application
ELISA (EIA), Immunocytochemistry, Immunohistochemistry-Paraffin(IHC-P), Dot Blot (DB), Immunoblotting (IB)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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Conjugation
Biotinylated
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About
Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Short name
Rabbit anti IgM (Fc specific), conjugated with
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Technique
Rabbit, anti, IgM, FC, antibody to, antibody Conjugates, IgMs
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Host
Rabbit, Rabbits
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Isotype
IgM, IgM
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Label
Biotin
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Species
Sheep, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
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Alternative name
production species: rabbit antibody to ovine Immunoglobulin M (fragment c specific), coupled including biotinilated
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Alternative technique
rabbit-anti, antibodies, immunoglobulin-m
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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