Goat anti Rat IgM biotin mu chain specific
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Catalog number
43R-IG071bt
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Price
Please ask
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Size
1 ml
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Category
Secondary Antibody
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Antibody Subtype
Conjugated Secondary Antibodies
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Area of research
Immunology
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Type of Immunogen
Goat anti-rat IgM (biotin) (mu chain specific) was raised in goat using rat IgM, Mu chain ..
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Raised in
Goat
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Specificity
Rat
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Cross Reactivity
Should be determined by the end user
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Conjugate Tag
Biotin
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Method of Purification
Goat anti Rat IgM (biotin) (mu chain sepcific) was purified by affinity chromatography.
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Form Buffer
Lyophilized from 0.01M Na3PO4, 0.25M NaCl, pH 7.6, with 15mg/ml BSA, and 0.05% NaN3.
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Storage
Store at 4 deg C.
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Shipping conditions
Blue Ice
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Tested for
ELISA; FC; ICC; IHC; WB
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Usage Recommendations
ELISA: 1:20,000-1:400,000, FC: 1:200-1:1,000, ICC: 1:500-1:5,000, IHC: 1:500-1:5,000, WB: 1:20,000-1:400,000
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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Conjugation
Biotinylated
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Latin name
Capra aegagrus hircus, Rattus norvegicus
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target
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Short name
Goat anti IgM mu chain specific
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Technique
anti, Goat, IgM, antibody to, goats, IgMs
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgM, IgM
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Label
Biotin
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Species
Rat, Goats, Rats
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Alternative name
Goat anti Rat IgM secondary antibody (biotin) (mu chain specific)
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Alternative technique
antibodies, caprine, immunoglobulin-m
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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