Rabbit anti Rat IgG1 IgG2a IgG2b IgG2c (heavy and light chains), conjugated with FITC
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Catalog number
MBS571396
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Price
Please ask
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Size
2 mL
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Products_type
Secondary Antibody
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Products_short_name
[Rabbit anti Rat IgG1 IgG2a IgG2b IgG2c (heavy and light chains)]
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Clonality
Polyclonal
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Reactivity
Rat
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Specificity
Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Rabbit antiSerum to Rat IgG, heavy and light chains.
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Storage_stability
Store at 4 degree C.
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Tested_application
ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), (In)direct Immunofluorescence (IF)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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About
Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Oryctolagus cuniculus, Rattus norvegicus
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Gene target
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Short name
Rabbit anti IgG1 IgG2a IgG2b IgG2c (heavy light chains), conjugated with FITC
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Technique
Rabbit, light, anti, FITC, antibody to, antibody Conjugates, Fluorescein
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Host
Rabbit, Rabbits
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Isotype
IgG1, IgG2a, IgG2b, IgG2c
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Label
FITC
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Species
Rat, Rats
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Alternative name
production species: rabbit antibody to Rat IgG1 IgG2a IgG2b IgG2c (heavy and light chains), coupled including fluorecein
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Alternative technique
rabbit-anti, antibodies, fluorescine
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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