Rabbit anti Rat IgG1 IgG2a IgG2b IgG2c (heavy and light chains), conjugated with FITC[Rabbit anti Rat IgG1 IgG2a IgG2b IgG2c (heavy and light chains)]

• Catalog number
  MBS571396
• Price
  Please ask
• Size
  NA

• Products_type
  Secondary Antibody
• Reactivity
  Rat
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• About
  Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Oryctolagus cuniculus, Rattus norvegicus

• Gene target
  IgG1   IgG2a   IgG2b   IgG2c   heavy   light   chains   conjugated   with   IgG1   IgG2a   IgG2b   IgG2c   heavy   light   chains  
• Short name
  Rabbit anti IgG1 IgG2a IgG2b IgG2c (heavy light chains), conjugated with FITC[Rabbit anti IgG1 IgG2a IgG2b IgG2c (heavy light chains)]
• Technique
  Rabbit, light, anti, FITC, antibody to, antibody Conjugates, Fluorescein
• Host
  Rabbit, Rabbits
• Isotype
  IgG1, IgG2a, IgG2b, IgG2c
• Label
  FITC
• Species
  Rat, Rats
• Alternative name
  production species: rabbit antibody to Rat IgG1 IgG2a IgG2b IgG2c (heavy and light chains), coupled including fluorecein[production species: rabbit antibody to Rat IgG1 IgG2a IgG2b IgG2c (heavy and light chains)]
• Alternative technique
  rabbit-anti, antibodies, fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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