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Products_type
Secondary Antibody
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Products_short_name
[IgG (H&L)]
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Products_name_syn
[Rabbit anti-Hamster IgG (H&L) - Affinity Pure]
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Reactivity
Hamster. Cross Reactivity: Based on IEP, no reactivity is observed to: non-immunoglobulin hamster serum immunoglobulins human serum proteins
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Specificity
Based on IEP, this antibody reacts with: heavy (gamma) chains on hamster IgG, light chains on all hamster immunoglobulins
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Purity
Affinity purified antibody is > 95% based on SDS-PAGE Affinity purified using solid phase Hamster IgG.
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Form
Clear, colorless liquid
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Concentration
1.50 mg/ml (E 1% at 280 nm = 13.0)
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Storage_stability
Store at 2-8 degree C. Shelf Life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
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Tested_application
ELISA (EIA), Immunohistochemistry (IHC)
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Test
A high affinity purification column was use to purify Rabbit anti- IgG (H L) - Pure, Conjugate min x serum proteins by MyBioSource by chromatographic size exclusion.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The ultrapure Rabbit anti- IgG (H L) - Pure, Conjugate min x serum proteins supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds. Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Biotinylated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Serum should be stored at +5°C
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Latin name
Oryctolagus cuniculus
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Group
Sera
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Gene target
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Short name
Rabbit anti- IgG (H L) - Pure, Conjugate min x serum proteins
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Technique
affinity, Rabbit, anti-, serum, anti, Pure, IgG, antibody to, antibodies, IgGs, pure products
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Host
Rabbit, Rabbits
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Isotype
IgG, Heavy & Light chains, IgG
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Label
Biotin
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Species
Human, Hamsters, Humans
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Alternative name
production species: rabbit Antibody toHamster Immunoglobulin G (heavy + light chain) - protein Pure, biotinilated Conjugate min x H. sapiens blood serum proteins
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Alternative technique
rabbit-anti, sera, antibodies, purified, immunoglobulins
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Tissue
serum
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