Goat anti-Rat IgG (H&L) - Affinity Pure, DyLight633 Conjugate min x w/bovine, goat, hamster, horse, human, mouse or rabbit IgG/serum
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Catalog numberMBS686258
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Price:
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Size5x1 mg
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Products_typeSecondary Antibody
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Products_short_name[IgG (H&L)]
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Products_name_syn[Goat anti-Rat IgG (H&L) - Affinity Pure]
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ReactivityRat. Cross Reactivity: Based on IEP, no reactivity is observed to: non-immunoglobulin rat serum proteins IgG from Goat, Hamster, Human, Mouse, Rabbit Serum from Bovine, Goat, Horse, Human, Mouse, Rabbit
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SpecificityBased on IEP, this antibody reacts with: heavy (gamma) chains on rat IgG, light chains on all rat immunoglobulins
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PurityAffinity purified antibody is > 95% based on SDS-PAGE Affinity purified using solid phase Rat IgG.
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FormLyophilized
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Concentration1.0 mg/ml (E 1% at 280 nm = 13.0)
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Storage_stabilityStore freeze-dried powder at 2-8 degree C. Shelf Life: Product is stable for up to 4 weeks at 2-8 degree C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20 degree C.
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Tested_applicationFlow Cytometry (FC/FACS), Immunofluorescence (IF), ELISA (EIA), Immunomicroscopy (IM)
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TestA high affinity purification column was use to purify Goat anti- IgG (H L) - Pure, DyLight633 Conjugate min x w/ , goat, , , , mouse rabbit IgG/serum by MyBioSource by chromatographic size exclusion. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The ultrapure Goat anti- IgG (H L) - Pure, DyLight633 Conjugate min x w/ , goat, , , , mouse rabbit IgG/serum supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds. Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Latin nameCapra aegagrus hircus, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
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PropertiesArmenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry. Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats. Serum should be stored at +5°C
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GroupSera
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Gene target
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Short nameGoat anti- IgG (H L) - Pure, DyLight633 Conjugate min x w/ , goat, , , , mouse rabbit IgG/serum
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Techniqueaffinity, Rabbit, anti-, Mouse, serum, anti, Goat, Pure, IgG, antibody to, antibodies, goats, IgGs, mouses, pure products
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HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
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IsotypeIgG, Heavy & Light chains, IgG
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SpeciesMouse, Bos Taurus genes are available form the Bovine Genome database. Goats, Hamsters, Horses, Humans, Mouses, Rats
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Alternative namecaprine Antibody toRat Immunoglobulin G (heavy + light chain) - protein Pure, DyLight633 Conjugate min x w/bovine, caprine, hamster, horse, H. sapiens, mouse and production species: rabbit Immunoglobulin G/blood serum
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Alternative techniquerabbit-anti, sera, murine, antibodies, purified, caprine, immunoglobulins
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Tissueserum
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data