Hamster Anti Mouse CD28 FITC
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Catalog number
MBS140138
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Price
457.1 USD
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Size
0.5 mg
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Products_type
Antibody
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Products_short_name
[CD28]
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Products_name_syn
[CD28 Antibody, FITC; CD28, Hamster Anti-Mouse, FITC; CD28 FITC]
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Other_names
[T-cell-specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28; CD28 antigen; CD_antigen: CD28]
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Products_gene_name
[CD28]
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Other_gene_names
[Cd28; Cd28]
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Clonality
Monoclonal
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Clone
[NYRmCD28]
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Purity
Protein-A.
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Concentration
1mg/ml in PBS (after reconstitution).
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Storage_stability
Lyophilized: store at 4 degree C. After reconstitution, if not intended for use within a month, aliquot and store at -20 degree C.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
CD28
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Short name
Anti Mouse CD28 FITC
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Technique
anti mouse, Mouse, anti, FITC, antibody to, anti mouse antibodies, Fluorescein, mouses
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Host
Hamster
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Isotype
IgG
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Label
FITC
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Species
Mouse, Hamsters, Mouses
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Alternative name
Hamster antibody to Mouse CD28 molecule fluorecein
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Alternative technique
murine, antibodies, fluorescine
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Alternative to gene target
CD28 molecule, Tp44, CD28 and IDBG-79183 and ENSG00000178562 and 940, identical protein binding, Cell surfaces, Cd28 and IDBG-159608 and ENSMUSG00000026012 and 12487, CD28 and IDBG-643371 and ENSBTAG00000007445 and 281050
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Gene info
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Identity
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Gene
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Long gene name
CD28 molecule
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Synonyms gene name
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1988-05-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Ig-like cell adhesion molecule family
- V-set domain containing
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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