MOUSE ANTI BOVINE CD2:FITC
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Catalog number
MBS216620
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Price
Please ask
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Size
5x100 Tests
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Products_type
Antibody
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Products_short_name
[CD2]
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Other_names
[CD2 molecule; CD2 molecule; cluster of differentiation 2]
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Products_gene_name
[CD2]
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Other_gene_names
[CD2]
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Clonality
Monoclonal
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Clone
[CC42]
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Specificity
This item recognises the bovine homologue of human CD2. The antibody inhibits rosetting with SRBC and stains cells in primary and secondary lymphoid organs in patterns consistent with those seen by human CD2 monoclonal antibodies.
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Form
FITC Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
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Concentration
IgG concentration 0.1mg/ml
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Storage_stability
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
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Tested_application
Flow cytometry (FC/FACS)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
CD2
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Short name
MOUSE ANTI CD2:FITC
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Technique
Mouse, anti, FITC, antibody to, Fluorescein, mouses
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Host
Mouse
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Isotype
IgG1
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Label
FITC
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Species
Bovine, Bos Taurus genes are available form the Bovine Genome database. Mouses
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Alternative name
MOUSE antibody to BOVINE CD2 molecule:fluorecein
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Alternative technique
murine, antibodies, fluorescine
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Alternative to gene target
CD2 molecule, LFA-2 and SRBC and T11, CD2 and IDBG-101417 and ENSG00000116824 and 914, protein homodimerization activity, Extracellular, Cd2 and IDBG-179684 and ENSMUSG00000027863 and 12481, CD2 and IDBG-634458 and ENSBTAG00000017256 and 497022
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Gene info
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Identity
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Gene
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Long gene name
CD2 molecule
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Synonyms gene
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Synonyms gene name
- CD2 antigen (p50), sheep red blood cell receptor
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GenBank acession
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Ig-like cell adhesion molecule family
- C2-set domain containing
- V-set domain containing
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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