MHC Class 1, H2, Kd, Dd (Biotin)

Products_type

Antibody
Products_short_name

[MHC Class 1, H2, Kd, Dd]
Products_name_syn

[Anti -MHC Class 1, H2, Kd, Dd (Biotin)]
Clonality

Monoclonal
Clone

[34-1-2S]
Reactivity

Mouse
Specificity

Recognizes an epitope in the alpha3 domain that is common to H-2Kd and H-2Dd. Binds to a common determinant in the alpha3 domains of H-2Kd and H-2Dd in the presence or absence of b2 microglobulin. It crossreacts with the alpha3 domain of H-2Kb.
Purity

Purified Purified
Form

Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to Biotin.
Storage_stability

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Tested_application

Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated

Gene target

MHC Class
Short name

MHC Class 1, H2, Kd, Dd ( )
Host

Mouse
Isotype

IgG2a,k
Label

Biotin
Alternative name

Major histocompabitility complex Class 1, H2, Kd, Dd (biotinilated)