MHC Class I H2Kk antibody biotin

Category

Primary Antibody
Antibody Subtype

Conjugated Monoclonal Antibodies
Area of research

Immunology
Raised in

Mouse
Isotypes

IgG1
Clone

AF3-12.1.3
Conjugate Tag

Biotin
Concentration

500 ug/ml
Form Buffer

Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.
Storage

Store at 2-8 deg C. Do not freeze. Protect from light.
Shipping conditions

Blue Ice
Tested for

FC
Assay Information

This eBio299Arm antibody has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood and can be used at the same concentration as the experimental antibody.
URL
More info
Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
French translation

anticorps

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data