Medium 199 w/ Earle's, L-Glutamine, HEPES (Powder, TC 199)[Medium 199 w/ Earle's, L-Glutamine, HEPES]

• Catalog number
  MBS653291
• Price
  Please ask
• Size
  NA

• Products_type
  Culture Media
• Buffer
  25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
• Description
  There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
• Group
  media

• Gene target
  199   Earle's   L-Glutamine   Powder   199   199   Earle's   L-Glutamine  
• Short name
  Medium 199 w/ Earle's, L-Glutamine, (Powder, TC 199)[Medium 199 w/ Earle's, L-Glutamine, ]
• Technique
  Medium, cell culture Media
• Alternative name
  Medium 199 w/ Earle's, L-Glutamine, HEPES (Powder, TC 199)[Medium 199 w/ Earle's, L-Glutamine, HEPES]
• Alternative technique
  cellculture
• Virus
  hhv

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products