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Description
Medium 199 With Earle's salts, 25mM HEPES buffer and L-Glutamine Without Sodium bicarbonate, is an off-white to creamish white, homogenous powder. It is a complex medium That was formulated specifically for nutritional studies on primary chick embryo fibroblasts in the absence of any additives. When supplemented with serum, It can be used for growth of a wide variety of cells. Presently, It is used for the maintenance of non-transformed cells, vaccine and virus production and primary explants of epithelial cells.
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Shelf life
Use before expiry date given on the product label.
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Storage
Store at 2-8°C away from bright light
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Appearance
Off-white to Creamish white, homogenous powder.
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PH without Sodium Bicarbonate
4.80 -5.40
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PH with Sodium Bicarbonate
6.60 -7.20
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Osmolality without Sodium Bicarbonate
265.00 -305.00
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Osmolality with Sodium Bicarbonate
310.00 -350.00
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Cultural Response
The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures.
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Endotoxin Content
NMT 5EU/ml
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Solubility
Clear solution at 15.6 gms/L.
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Uses
Laboratory chemicals, Manufacture of substances
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Personal Protection
Use personnel protective equipment. Wear disposable gloves, dust mask and eye protection. Avoid dust formation.
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Toxicological Information
NA
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Ordering
To order Medium 199 With Earle's salts, 25mM HEPES buffer and L-Glutamine Without Sodium bicarbonate, please use the Cat. Nr.AT094-10X5L and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for Medium 199 With Earle's salts, 25mM HEPES buffer and L-Glutamine Without Sodium bicarbonate
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Additional description
Buffering solutions are useful to keep the pH range sable when using this reagent of Himedia. There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
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Buffer
25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
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Group
media
