Ly49I (FITC)

• Catalog number
  MBS602595
• Price
  Please ask
• Size
  5x0.5 mg

• Products_type
  Antibody
• Products_short_name
  [Ly49I]
• Products_name_syn
  [Anti -Ly49I (FITC)]
• Other_names
  [Ly49I receptor]
• Clonality
  Monoclonal
• Clone
  [5K29]
• Reactivity
  Mouse
• Specificity
  Recognizes the B6 allele of the Ly49I receptor. Reacts with the B6 allele of Ly49I, a member of the lectin-like homodimeric Ly49 family of cell surface receptors.
• Purity
  Purified Purified
• Form
  Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to FITC.
• Storage_stability
  May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Tested_application
  Flow Cytometry (FC/FACS)
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody

• Gene target
  Ly49I  
• Short name
  Ly49I (FITC)
• Technique
  FITC, Fluorescein
• Host
  Mouse
• Isotype
  IgG1,k
• Label
  FITC
• Alternative name
  Ly49I (fluorecein)
• Alternative technique
  fluorescine

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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