Ly49I (FITC)

• Catalog number
  MBS602595
• Price
  Please ask
• Size
  0.5 mg

• Products_type
  Antibody
• Products_short_name
  [Ly49I]
• Products_name_syn
  [Anti -Ly49I (FITC)]
• Other_names
  [Ly49I receptor]
• Clonality
  Monoclonal
• Clone
  [5K29]
• Reactivity
  Mouse
• Specificity
  Recognizes the B6 allele of the Ly49I receptor. Reacts with the B6 allele of Ly49I, a member of the lectin-like homodimeric Ly49 family of cell surface receptors.
• Purity
  Purified Purified
• Form
  Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to FITC.
• Storage_stability
  May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Tested_application
  Flow Cytometry (FC/FACS)
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody

• Gene target
  Ly49I  
• Short name
  Ly49I (FITC)
• Technique
  FITC, Fluorescein
• Host
  Mouse
• Isotype
  IgG1,k
• Label
  FITC
• Alternative name
  Ly49I (fluorecein)
• Alternative technique
  fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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