IgG, Sheep Isotype Control (Biotin)
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Catalog number
MBS655731
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Price
Please ask
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Size
1 mg
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Products_type
Isotype Control
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Products_short_name
[IgG, Sheep Isotype Control]
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Purity
Purified Purified
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Form
Supplied as a liquid in PBS, sodium azide. Labeled with Biotin.
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Storage_stability
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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Tested_application
ELISA (EL/EIA), Flow Cytometry (FC/FACS)
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
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Conjugation
Biotinylated
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Description
Isotype or positive controls by peptides, antibodies and deactivated samples.
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Group
isotypes
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Short name
IgG, Isotype Control ( )
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Technique
Control, Isotype, IgG, controls, IgGs, Isotypes and controls
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Host
Normal sheep serum
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Isotype
IgG, IgG
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Label
Biotin
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Species
Sheep, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
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Alternative name
Immunoglobulin G, ovine isoform reference (biotinilated)
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Alternative technique
control, controls, immunoglobulins
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Tissue
control
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Product images
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