IgG, Rat Isotype Control (FITC)

#

• Catalog number
  MBS655750
• Price:

  1800.53 USD

  Ask for price
• Size
  5x0.5 mg

# #

• Products_type
  Isotype Control
• Products_short_name
  [IgG, Rat Isotype Control]
• Purity
  Purified Purified
• Form
  Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to FITC.
• Storage_stability
  May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Tested_application
  ELISA (EL/EIA), Flow Cytometry (FC/FACS)
• Description
  Isotype or positive controls by peptides, antibodies and deactivated samples.
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
• Conjugation
  Anti-FITC Antibody
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Group
  isotypes
• Latin name
  Rattus norvegicus

#

• Short name
  IgG, Isotype Control (FITC)
• Technique
  Control, Isotype, FITC, IgG, controls, Fluorescein, IgGs, Isotypes and controls
• Host
  Normal rat serum
• Isotype
  IgG, IgG
• Label
  FITC
• Species
  Rat, Rats
• Alternative name
  Immunoglobulin G, Rat isoform reference (fluorecein)
• Alternative technique
  control, controls, fluorescine, immunoglobulins
• Tissue
  control

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products