IgG F(ab')2, Feline (FITC)

• Catalog number
  MBS654941
• Price
  Please ask
• Size
  5x1 mg

• Products_type
  Immunoglobulin
• Products_short_name
  [IgG F(ab')2, Feline]
• Specificity
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Cat IgG, anti-Cat IgG F(ab')2 and anti-Cat Serum. No reaction was observed against
• Purity
  Highly Purified Purified by ion exchange chromatography.
• Form
  Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA, 0.1% sodium azide. Conjugated to Fluorescein isothiocyanate (FITC). Free FITC removed by solid phase adsorption.
• Storage_stability
  May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C or colder. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Tested_application
  Western Blot (WB), Immunofluorescence (IF)
• Group
  f(ab')2s
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

• Short name
  IgG F(ab')2, Feline (FITC)
• Technique
  F(ab')2, Feline, FITC, IgG,  F(ab')2 antibody fragments are the antigen binding variable regions obtained by enzymatic cutting of the parent IgG at neutral ph. felines, Fluorescein, IgGs
• Isotype
  IgG, F(ab')2 Fragment Affinity-Purified Secondary Antibodies, IgG
• Label
  FITC
• Species
  Sometimes til 5% of an area felines have specific IgGs for the feline retroviral feLV infecting Felidae. Also distemper and leukemia virusses apear in cats and have their specific IgG antibodies that are very stable. All cats used for producing immunoglobulins are screened and tested virus negative. Cat seras have usually higher IgG tirters than dog serum. Feline range = 10 to 20 mg/ml IgGs.
• Alternative name
  Immunoglobulin G F(Antibody')2, Feline (fluorecein)
• Alternative technique
  secondairy, cats, fluorescine, immunoglobulins

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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