IgG F(ab')2, Feline (HRP)
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Catalog number
MBS654768
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Price
Please ask
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Size
1 mg
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Products_type
Immunoglobulin
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Products_short_name
[IgG F(ab')2, Feline]
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Purity
Highly Purified Purified by ion exchange chromatography. IEP Assay shows a single precipitin arc against source serum protein and IgG.
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Form
Lyophilized in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Conjugated to horseradish peroxidase (HRP).
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Storage_stability
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C or colder. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested_application
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
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Group
f(ab')2s
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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Conjugation
HRP Conjugated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Short name
IgG F(ab')2, Feline ( )
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Technique
F(ab')2, Feline, IgG, F(ab')2 antibody fragments are the antigen binding variable regions obtained by enzymatic cutting of the parent IgG at neutral ph. felines, IgGs
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Isotype
IgG, F(ab')2 Fragment Affinity-Purified Secondary Antibodies, IgG
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Label
HRP
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Species
Sometimes til 5% of an area felines have specific IgGs for the feline retroviral feLV infecting Felidae. Also distemper and leukemia virusses apear in cats and have their specific IgG antibodies that are very stable. All cats used for producing immunoglobulins are screened and tested virus negative. Cat seras have usually higher IgG tirters than dog serum. Feline range = 10 to 20 mg/ml IgGs.
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Alternative name
Immunoglobulin G F(Antibody')2, Feline (horseradish peroxidase)
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Alternative technique
secondairy, cats, immunoglobulins
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