HLA Class 1 Antigen A9 (BSA, Azide & Glycerol Free) (FITC)

  • Catalog number
    MBS604222
  • Price
    Please ask
  • Size
    0.25 mL
  • Products_type
    Antibody
  • Products_short_name
    [HLA Class 1 Antigen A9]
  • Products_name_syn
    [Anti -HLA Class 1 Antigen A9 (BSA, Azide & Glycerol Free) (FITC)]
  • Other_names
    [HLA-A9HH antigen; HLA class I histocompatibility antigen, A-68 alpha chain; HLA class I histocompatibility antigen, A-1 alpha chain; antigen presenting molecule; leukocyte antigen class I-A; MHC class I antigen HLA-A heavy chain; major histocompatibility complex, class I, A; Aw-68; HLA class I histocompatibility antigen, A-28 alpha chain; MHC class I antigen A*68]
  • Other_gene_names
    [HLA-A; HLA-A; HLAA; HLAA]
  • Clonality
    Monoclonal
  • Clone
    [0.L.1]
  • Reactivity
    Human
  • Specificity
    Reacts to HLA Class 1 Antigen-A9.
  • Purity
    Highly Purified Purified by Ion Exchange chromatography.
  • Form
    Supplied as a liquid in PBS. No stabilizing proteins added. No preservative added. Labeled with fluorescein isothiocyanate (FITC). Also available with BSA & Azide. See H6098-23A.
  • Storage_stability
    May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
  • Tested_application
    Flow Cytometry (FC/FACS), Immunofluorescence (IF)
  • Description
    Antigens are peptides or recombinant or native dependent on the production method. Sodium azide NaN3 is often used to prevent antibody degradation in 0,02% W/V concentrations. MyBioSource supplies azide free antibodies on request.
  • Properties
    This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • Gene target
    HLA   Class   BSA   Glycerol   Free  
  • Gene symbol
    HLA-DPA2, HLA-DOA, HLA-DQA1, HLA-N, HLA-DQA2, HLA-DPB1, HLA-DQB2, HLA-W, HLA-DRA, HLA-S
  • Short name
    HLA Class 1 Antigen A9 (BSA, & Glycerol Free) (FITC)
  • Technique
    antigen, FITC, antigenes, Fluorescein
  • Host
    Mouse
  • Isotype
    IgG2b
  • Label
    FITC
  • Alternative name
    Human leukocyte antigen Class 1 protein A9 (cow serum albumin, Azide & Glycerol Free) (fluorecein)
  • Alternative technique
    antigenes, fluorescine
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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