HEPES, Free Acid[HEPES, free acid ]
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Catalog numberMBS545255
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PricePlease ask
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Size1 kg
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Products_typeBiochemical
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Products_gene_name[HEPES]
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Buffer25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
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Gene target
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Gene symbolFFAR2, FFAR1, FFAR3
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Short name, Free Acid[ , free acid ]
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Alternative nameHEPES, Free Acid[HEPES, free acid ]
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Gene info
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Identity
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Gene
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Long gene namefree fatty acid receptor 2
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Synonyms gene
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Synonyms gene name
- G protein-coupled receptor 43
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-10-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Free fatty acid receptors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namefree fatty acid receptor 1
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Synonyms gene
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Synonyms gene name
- G protein-coupled receptor 40
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-10-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Free fatty acid receptors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namefree fatty acid receptor 3
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Synonyms gene
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Synonyms gene name
- G protein-coupled receptor 41
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-10-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Free fatty acid receptors
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VEGA ID