HEPES Free acid

Category

Small Molecules
Clonality

N/A
Raised in

N/A
Target

HEPES Free
Immunogen

see included datasheet or contact us
Species reactivity

Human, Mouse, Rat (for other species please inquire)
Purification

Affinity purified
Applications

WB (for other applications please contact us)
Stock availability

Shipped within 5-10 working days.
Storage conditions

Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
Footnote

This product is for research use only.
Other products
Buffer

25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.