Granulocyte Colony Stimulating Factor, Recombinant, Mouse, Western Blot Positive Control (GCSF, Granulocyte Colony-stimulating Factor, G-CSF CSF3)[Granulocyte Colony Stimulating Factor, Recombinant, Mouse, Western Blot Positive Control]

• Catalog number
  MBS650147
• Price
  Please ask
• Size
  NA

• Products_type
  Recombinant Protein
• Test
  Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Description
  Colonies can be formed by stimulating factors or recombinant GM-CSF and CSFs activity expressed in Units compared to a standard. Isotype or positive controls by peptides, antibodies and deactivated samples. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. Positive controls are the same as the target vector or antibody or protein and can be spiked to the sample before the analysis starts.
• Gene
  Granulocyte-colony stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream. Functionally, it is a cytokine and hormone, a type of colony-stimulating factor, and is produced by a number of different tissues. The pharmaceutical analogs of naturally occurring G-CSF are called filgrastim and lenograstim.
• Latin name
  Mus musculus
• Group
  positif, recombinants
• Source
  Recombinants or rec. proteins

• Gene target
  Granulocyte   Colony   Stimulating   Factor   GCSF   Granulocyte   Colony-stimulating   Factor   G-CSF   CSF3   [Granulocyte   Colony   Stimulating   Factor  
• Gene symbol
  CSF3, IGHV3-71, HLA-G, NCAPG, FOLR3, CSF2, SNRPG, GNAO1, CSF1, SFTA2
• Short name
  Granulocyte Colony Stimulating Factor, Recombinant, Mouse, Blot Positive Control (GCSF, Granulocyte Colony-stimulating Factor, G-CSF CSF3)[Granulocyte Colony Stimulating Factor, Recombinant, Mouse, Blot Positive Control]
• Technique
  Western Blot, Recombinant, Positive, Control, Mouse, Blot, western Blots, controls, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Host
  E Coli
• Species
  Mouse, Mouses
• Alternative name
  Granulocyte Colony Stimulating Factor, Rec., Mouse, Western Blot Positive reference (GCSF, Granulocyte Colony-stimulating Factor, G-CSF colony stimulating factor 3 (granulocyte))[Granulocyte Colony Stimulating Factor, Rec., Mouse, Western Blot Positive reference]
• Alternative technique
  rec, controls, murine, western
• Alternative to gene target
  colony stimulating factor 3 (granulocyte), C17orf33 and CSF3OS and GCSF, CSF3 and IDBG-46887 and ENSG00000108342 and 1440, enzyme binding, Extracellular, Csf3 and IDBG-210740 and ENSMUSG00000038067 and 12985, CSF3 and IDBG-639529 and ENSBTAG00000021462 and 281096
• Tissue
  control, granulocyte

Gene info

• Identity
  HGNC:2438
• Gene
  CSF3
• Long gene name
  colony stimulating factor 3
• Synonyms gene
  • GCSF
  • G-CSF
  • C17orf33
• Synonyms gene name
  • chromosome 17 open reading frame 33
  • colony stimulating factor 3 (granulocyte)
• Synonyms
  • MGC45931
• Synonyms name
  • granulocyte colony stimulating factor
  • pluripoietin
  • filgrastim
  • lenograstim
• Locus
  17q21.1
• Discovery year
  2001-06-22
• Entrez gene record
  1440
• Pubmed identfication
  • 3499671
  • 3501046
• RefSeq identity
  • NM_172220
• Classification
  • Receptor ligands
• VEGA ID
  OTTHUMG00000133247

Gene info

• Identity
  HGNC:5621
• Gene
  IGHV3-71
• Long gene name
  immunoglobulin heavy variable 3-71 (pseudogene)
• Synonyms gene
  • IGHV3-G
• Synonyms gene name
  • immunoglobulin heavy variable 3-71
  • immunoglobulin heavy variable 3-G pseudogene (provisional)
  • immunoglobulin heavy variable 3-G (provisional)
  • immunoglobulin heavy variable 3-71 pseudogene
• Synonyms
  • IGHV3-g
• GenBank acession
  • AB019437
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28411
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000151881

Gene info

• Identity
  HGNC:4964
• Gene
  HLA-G
• Long gene name
  major histocompatibility complex, class I, G
• Synonyms gene name
  • HLA-G histocompatibility antigen, class I, G
• Synonyms name
  • b2 microglobulin
• Locus
  6p22.1
• Discovery year
  1990-09-10
• Entrez gene record
  3135
• RefSeq identity
  • NM_002127
• Classification
  • C1-set domain containing
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000031157
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:24304
• Gene
  NCAPG
• Long gene name
  non-SMC condensin I complex subunit G
• Synonyms gene name
  • non-SMC condensin I complex, subunit G
• Synonyms
  • FLJ12450
  • hCAP-G
  • CAP-G
  • YCG1
• Synonyms name
  • chromosome condensation protein G
• GenBank acession
  • AF331796
• Locus
  4p15.31
• Discovery year
  2006-09-04
• Entrez gene record
  64151
• Pubmed identfication
  • 10910072
  • 11136719
• RefSeq identity
  • NM_022346
• Classification
  • Armadillo like helical domain containing
  • Condensin I subunits
• VEGA ID
  OTTHUMG00000128539

Gene info

• Identity
  HGNC:3795
• Gene
  FOLR3
• Long gene name
  folate receptor gamma
• Synonyms gene name
  • folate receptor 3 (gamma)
  • folate receptor 3
• Synonyms
  • FR-G
  • FRγ
• GenBank acession
  • U08471
• Locus
  11q13.4
• Discovery year
  1997-09-05
• Entrez gene record
  2352
• Pubmed identfication
  • 8110752
• RefSeq identity
  • NM_000804
• VEGA ID
  OTTHUMG00000167870

Gene info

• Identity
  HGNC:2434
• Gene
  CSF2
• Long gene name
  colony stimulating factor 2
• Synonyms gene name
  • colony stimulating factor 2 (granulocyte-macrophage)
• Synonyms
  • GM-CSF
  • GMCSF
• Synonyms name
  • sargramostim
  • molgramostim
  • granulocyte-macrophage colony stimulating factor
• GenBank acession
  • M11734
• Locus
  5q31.1
• Discovery year
  2001-06-22
• Entrez gene record
  1437
• Pubmed identfication
  • 3898082
  • 2999978
• RefSeq identity
  • NM_000758
• VEGA ID
  OTTHUMG00000059637

Gene info

• Identity
  HGNC:11163
• Gene
  SNRPG
• Long gene name
  small nuclear ribonucleoprotein polypeptide G
• Synonyms
  • Sm-G
• GenBank acession
  • X85373
• Locus
  2p13.3
• Discovery year
  1990-06-13
• Entrez gene record
  6637
• Pubmed identfication
  • 7744013
• Classification
  • Sm spliceosomal proteins
• VEGA ID
  OTTHUMG00000129670

Gene info

• Identity
  HGNC:4389
• Gene
  GNAO1
• Long gene name
  G protein subunit alpha o1
• Synonyms gene name
  • guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
• Synonyms
  • G-ALPHA-o
• Locus
  16q13
• Discovery year
  1988-04-24
• Entrez gene record
  2775
• Pubmed identfication
  • 1899283
  • 11395521
• RefSeq identity
  • NM_020988
• Classification
  • G protein subunits alpha, group i
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000133241

Gene info

• Identity
  HGNC:2432
• Gene
  CSF1
• Long gene name
  colony stimulating factor 1
• Synonyms gene name
  • colony stimulating factor 1 (macrophage)
• Synonyms
  • M-CSF
  • MCSF
  • MGC31930
• Synonyms name
  • macrophage colony stimulating factor 1
• GenBank acession
  • BC021117
• Locus
  1p13.3
• Discovery year
  1986-01-01
• Entrez gene record
  1435
• Pubmed identfication
  • 1540160
• RefSeq identity
  • NM_000757
• VEGA ID
  OTTHUMG00000011646

Gene info

• Identity
  HGNC:18386
• Gene
  SFTA2
• Long gene name
  surfactant associated 2
• Synonyms gene
  • SFTPG
• Synonyms gene name
  • surfactant associated protein G
• Synonyms
  • SP-G
• GenBank acession
  • AY102070
• Locus
  6p21.33
• Discovery year
  2002-05-01
• Entrez gene record
  389376
• Pubmed identfication
  • 22768197
  • 23094088
• RefSeq identity
  • NM_205854
• VEGA ID
  OTTHUMG00000031183

MeSH Data

• Name
  Colony-Forming Units Assay
• Concept
  Scope note: A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.
• Tree numbers
  • E01.370.225.500.383
  • E05.200.500.383
  • E05.242.383
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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