Granulocyte Colony Stimulating Factor, Recombinant, Human, Western Blot Positive Control (G-CSF, GCSF, C17orf33, Colony Stimulating Factor 3 (Granulocyte), CSF3, CSF3OS, Filgrastim, Lenograstim, MGC45931, Pluripoietin)
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Catalog numberMBS650565
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PricePlease ask
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Size5x0.1 mL
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Products_typeRecombinant Protein
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Products_short_name[Granulocyte Colony Stimulating Factor, Recombinant, Human, Western Blot Positive Control]
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Products_name_syn[G-CSF; GCSF; C17orf33; Colony Stimulating Factor 3 (Granulocyte); CSF3; CSF3OS; Filgrastim; Lenograstim; MGC45931; Pluripoietin]
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Other_names[granulocyte colony stimulating factor]
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PurityPurified (Highly Purified (~95%))
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FormSupplied as a liquid in SDS-PAGE sample buffer (reduced).
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Storage_stabilityMay be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.Shipping Temperature: Blue Ice
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Tested_applicationWestern Blot
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TestWestern Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.
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DescriptionColonies can be formed by stimulating factors or recombinant GM-CSF and CSFs activity expressed in Units compared to a standard. Isotype or positive controls by peptides, antibodies and deactivated samples. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. Positive controls are the same as the target vector or antibody or protein and can be spiked to the sample before the analysis starts.
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GeneGranulocyte-colony stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream. Functionally, it is a cytokine and hormone, a type of colony-stimulating factor, and is produced by a number of different tissues. The pharmaceutical analogs of naturally occurring G-CSF are called filgrastim and lenograstim.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Grouppositif, recombinants
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SourceRecombinants or rec. proteins
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Gene target
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Gene symbolCSF3, IGHV3-71, HLA-G, NCAPG, CSF2, FOLR3, SNRPG, GNAO1, CSF1, SFTA2
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Short nameGranulocyte Colony Stimulating Factor, Recombinant, , Blot Positive Control (G-CSF, GCSF, C17orf33, Colony Stimulating Factor 3 (Granulocyte), CSF3, CSF3OS, Filgrastim, Lenograstim, MGC45931, Pluripoietin)
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TechniqueWestern Blot, Recombinant, Positive, Control, Blot, western Blots, controls, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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HostE Coli
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SpeciesHuman, Humans
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Alternative nameGranulocyte Colony Stimulating Factor, Rec., H. sapiens, Western Blot Positive reference (G-CSF, GCSF, C17orf33, Colony Stimulating Factor 3 (Granulocyte), colony stimulating factor 3 (granulocyte), CSF3OS, Filgrastim, Lenograstim, MGC45931, Pluripoietin)
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Alternative techniquerec, controls, western
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Alternative to gene targetcolony stimulating factor 3 (granulocyte), C17orf33 and CSF3OS and GCSF, CSF3 and IDBG-46887 and ENSG00000108342 and 1440, enzyme binding, Extracellular, Csf3 and IDBG-210740 and ENSMUSG00000038067 and 12985, CSF3 and IDBG-639529 and ENSBTAG00000021462 and 281096
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Tissuecontrol, granulocyte
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Gene info
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Identity
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Gene
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Long gene namecolony stimulating factor 3
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Synonyms gene
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Synonyms gene name
- chromosome 17 open reading frame 33
- colony stimulating factor 3 (granulocyte)
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Synonyms
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Synonyms name
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Receptor ligands
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-71 (pseudogene)
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Synonyms gene
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Synonyms gene name
- immunoglobulin heavy variable 3-71
- immunoglobulin heavy variable 3-G pseudogene (provisional)
- immunoglobulin heavy variable 3-G (provisional)
- immunoglobulin heavy variable 3-71 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, G
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Synonyms gene name
- HLA-G histocompatibility antigen, class I, G
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Synonyms name
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Locus
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Discovery year1990-09-10
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Entrez gene record
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namenon-SMC condensin I complex subunit G
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Synonyms gene name
- non-SMC condensin I complex, subunit G
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2006-09-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Armadillo like helical domain containing
- Condensin I subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecolony stimulating factor 2
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Synonyms gene name
- colony stimulating factor 2 (granulocyte-macrophage)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namefolate receptor gamma
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Synonyms gene name
- folate receptor 3 (gamma)
- folate receptor 3
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-09-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesmall nuclear ribonucleoprotein polypeptide G
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Synonyms
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GenBank acession
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Locus
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Discovery year1990-06-13
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Entrez gene record
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Pubmed identfication
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Classification
- Sm spliceosomal proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameG protein subunit alpha o1
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Synonyms gene name
- guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
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Synonyms
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Locus
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Discovery year1988-04-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- G protein subunits alpha, group i
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecolony stimulating factor 1
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Synonyms gene name
- colony stimulating factor 1 (macrophage)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesurfactant associated 2
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Synonyms gene
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Synonyms gene name
- surfactant associated protein G
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-05-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.
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Tree numbers
- E01.370.225.500.383
- E05.200.500.383
- E05.242.383
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data